RPOC2_WHEAT
ID RPOC2_WHEAT Reviewed; 1479 AA.
AC Q9XPS9;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Triticum aestivum (Wheat).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Chinese Spring;
RA Matsuoka Y., Tsunewaki K., Ohnishi Y.;
RT "Molecular analysis of a 21.1-kb fragment of wheat chloroplast DNA bearing
RT RNA polymerase subunit (rpo) genes.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring;
RA Ogihara Y., Isono K., Kojima T., Endo A., Hanaoka M., Shiina T.,
RA Terachi T., Utsugi S., Murata M., Mori N., Takumi S., Ikeo K., Gojobori T.,
RA Murai R., Murai K., Matsuoka Y., Ohnishi Y., Tajiri H., Tsunewaki K.;
RT "Chinese spring wheat (Triticum aestivum L.) chloroplast genome: complete
RT sequence and contig clones.";
RL Plant Mol. Biol. Rep. 18:243-253(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AB027572; BAA78042.1; -; Genomic_DNA.
DR EMBL; AB042240; BAB47026.1; -; Genomic_DNA.
DR RefSeq; NP_114251.1; NC_002762.1.
DR AlphaFoldDB; Q9XPS9; -.
DR STRING; 4565.EPlTAEP00000010025; -.
DR PRIDE; Q9XPS9; -.
DR GeneID; 803165; -.
DR KEGG; taes:803165; -.
DR eggNOG; ENOG502QPYA; Eukaryota.
DR HOGENOM; CLU_000524_1_0_1; -.
DR Proteomes; UP000019116; Chloroplast.
DR Genevisible; Q9XPS9; TA.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW Transferase; Zinc.
FT CHAIN 1..1479
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067953"
FT REGION 618..640
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 663..756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 678..706
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..746
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1479 AA; 170045 MW; 4DFB5363CB516FB2 CRC64;
MAERANLVFH NKEIDGTGMK RLISRLIDHF GMGYTSHILD QLKTLGFHQA TTTSISLGIE
DLLTIPSKGW LVQDAEQQSF LLEKHYYYGA VHAVEKLRQS VEIWYATSEY LKQEMNSNFR
ITDPSNPVYL MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTADAG YLTRRLVEVV QHIIVRRRDC GTIRGISVSP QNGMTEKLFV
QTLIGRVLAD DIYIGSRCIA ARNQDIGIGL VNRFITAFRA QPFRAQPIYI RTPFTCRSTS
WICQLCYGRS PTHSDLVELG EAVGIIAGQS IGEPGTQLTL RTFHTGGVFT GGTADLVRSP
SNGKIKFNEN LVHPTRTRHG QPAFLCYIDL HVTIQSQDIL YSVNIPSKSL ILVQNDQYVK
SEQVIAEIRA GTSTLHFKER VQKHIYSESD GEMHWSTDVY HAPEYQYGNL RRLPKTSHLW
ILSVSMCRSS IASFSLHKDQ DQMNTYGKKD REILDYSTSD RIMSNGHWNF IYPSIFQDNS
DLLAKKRRNR FVIPLQYHQE QEKELISCFG ISIEIPLMGV LRRNTIFAYF DDPRYRKDKK
GSGIVKFRYR TLEEEYRTRA EDSEEEYETL EDEYRTREDE YEYETLEESK YGILEDEYEY
ETLEDEYGSP ENEYGNPENE YRTLEKDSEE EYGSPESKYR TQEDEYGTIE EDSEDEYGSP
GESAEEKYGT LEEDSEEDSE DEYESPEEDS ILKKEGLIEH RGTKEFSLKY QKEVDRFFFI
LQELHILPRS SSLKILDNSI IGVDTQLTKN TRSRLGGLVR VKRKKSHTEL KIFSGDIHFP
EEADKILGGC LIPPERQKKD SKESKKRKNW VYVQRKKILK SKEKYFVSVR PTVAYEMDEG
RNLATLFPQD LLQEENNLQI RLVNFISHEN SKLTQRIYHT NSQFVRTCLV VNWEQEEKEK
AGASLVEVRA NDLIRDFLRI ELVKSTISYT RKRYDRTSAG PIPHNRLDRA NINSFYSKAK
IESLSQHPEA IGTLLNRNKE YHSLMILSAS NCSRIGLFKN SKHPNAIKEW NPRIPIREIF
GPLGAIVASI SHFSSSYYLL THNKILLKKY LFVDNLKQTF QVLQELKYSL IDENKRISNF
DSNIMLDPFL LNCHFVHHDS WEETLAIIHL GQFICENVCL FKSHIKKSGQ IFIVNMNSFV
IRAAKPYLAT TGATVNGHYG EILYKGDRLV TFIYEKSRSS DITQGLPKVE QIFEARSIDS
LSPNLERRIE DWNERIPRIL GVPWGFLIGA ELTIAQSRIS LVNKIQKVYR SQGVQIHNRH
IEIIIRQVTS KVRVSEDGMS NVFSPGELIG LLRAERAGRA LDESIYYRAI LLGITRASLN
TQSFISEASF QETARVLAKA ALRGRIDWLK GLKENVVLGG IIPVGTGFQK FVHRSPQDKN
LYFEIKKKNL FASEMRDFLF LHTELVSSDS DVTNNFYET
