RPOC_FRATH
ID RPOC_FRATH Reviewed; 1417 AA.
AC Q2A1M8;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; OrderedLocusNames=FTL_1743;
OS Francisella tularensis subsp. holarctica (strain LVS).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=376619;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVS;
RA Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S.,
RA Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R.,
RA Garcia E.;
RT "Complete genome sequence of Francisella tularensis LVS (Live Vaccine
RT Strain).";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01322};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
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DR EMBL; AM233362; CAJ80182.1; -; Genomic_DNA.
DR RefSeq; WP_003017226.1; NZ_CP009694.1.
DR PDB; 6WMP; EM; 2.98 A; D=1-1417.
DR PDB; 6WMR; EM; 3.46 A; D=1-1417.
DR PDB; 6WMT; EM; 4.43 A; D=1-1417.
DR PDBsum; 6WMP; -.
DR PDBsum; 6WMR; -.
DR PDBsum; 6WMT; -.
DR AlphaFoldDB; Q2A1M8; -.
DR SMR; Q2A1M8; -.
DR KEGG; ftl:FTL_1743; -.
DR OMA; YRNIRVE; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Transcription; Transferase; Zinc.
FT CHAIN 1..1417
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000240802"
FT BINDING 68
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 83
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 86
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 458
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 460
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 462
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 811
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 884
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 891
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 894
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT STRAND 17..22
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 25..31
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 32..35
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 63..66
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 84..87
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 93..97
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 116..119
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 121..127
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 130..137
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 140..146
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 149..151
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 162..164
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 165..169
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 172..177
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 181..188
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 192..203
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 211..228
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 236..242
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 254..256
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 257..260
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 265..282
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 287..306
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 309..312
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 319..321
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 325..327
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 336..339
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 340..342
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 344..354
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 363..367
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 368..374
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 376..385
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 392..400
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 406..413
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 414..416
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 419..422
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 429..431
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 432..447
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 449..451
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 452..455
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 459..461
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 463..467
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 472..480
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 484..486
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 491..494
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 504..511
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 519..522
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 524..527
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 528..535
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 536..538
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 545..555
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 561..571
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 572..575
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 576..580
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 587..590
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 596..610
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 612..631
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 632..634
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 639..642
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 648..667
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 674..676
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 680..694
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 696..699
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 701..706
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 709..714
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 720..724
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 726..728
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 731..738
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 760..762
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 766..770
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 773..784
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 787..800
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 806..809
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 817..819
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 825..827
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 833..835
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 836..838
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 840..843
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 848..850
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 852..854
FT /evidence="ECO:0007829|PDB:6WMR"
FT TURN 862..864
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 866..870
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 876..878
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 881..883
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 887..891
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 892..895
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 899..904
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 911..916
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 917..919
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 921..925
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1125..1132
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1156..1158
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1168..1170
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1207..1209
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1210..1212
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1213..1229
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1230..1233
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1238..1240
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1242..1245
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1246..1248
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1251..1256
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1258..1260
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1265..1267
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1268..1274
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1275..1277
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1278..1281
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1282..1284
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1290..1292
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1299..1302
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1307..1311
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1316..1325
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1329..1331
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1335..1340
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1349..1363
FT /evidence="ECO:0007829|PDB:6WMP"
SQ SEQUENCE 1417 AA; 157387 MW; 9466D0F0DB6F6F3D CRC64;
MNNGILHQNY NSKKFDIIKI SLASPEVIRS WSHGEVKKPE TINYRTFKPE RDGLFCAKIF
GPIKDYECLC GKYKRLKHRG VVCERCGVEV EQAKVRRERM GHIDLVCPVV HIWYLKSLPS
RIGLFLDMPL KNVEKVLYFE SYIVTDPGMT PLEKKQLLTD EEYAEALENY GYEFEASMGA
EAIRDLLADT DIESEIELLQ AECEESKSTA KKEKAIKRLR LLETFQASGN KPEWMVMTVL
PVLPPDLRPL VPIEGGRFAT SDLNDLYRRV INRNNRLKKL LDLNAPDIIV RNEKRMLQEA
VDALLDNGRR GRAVTGSNKR PLKSLADMIK GKQGRFRQNL LGKRVDYSGR SVITVGPSLR
LHECGLPKKM ALELFKPFVY SKLRLGGHAT TIKQAKRMVE LEEAVVWDIL ETVINEHPVL
LNRAPTLHRL GIQAFEPRLI EGKAIQLHPL VCAAFNADFD GDQMAVHVPL TVESQLEARV
LMMSTNNILS PASGQPIITP TQDIVLGLYY ITREKEGARG EGKLFSSYED VSRAYNSGTI
DIHAKIKLRI DRQVFDTKGN TYNEKGVVNT TVGRALLLNI LPEGLSFSLL NKVLVKKEIS
KIINQAFRVL GGKATVVLAD KLMYAGFKYS TLSGVSVGVD DMTIPDNKEA KIEEAEKEIK
QITEQYQSSL ITENERYNNI INIWSKTSDE VGASMMDAIS KDTVSINGEK KEIESFNSVY
MMAKSGARGS YNQMRQLAGM RGLMAKPDGT MIETAITANF REGLSVLQYF TSTHGARKGL
ADTALKTANA GYLTRRLVDV AQDLVVIEED CGTDDGLMFS AIVEDGEVKV PLVERALGRT
LAADVVTEKG VVLLEAGTLL DENLVELLDD NGIDMIKVRS PITCKTRRGL CAKCYGRDLA
RERQVNVGES VGVIAAQSIG EPGTQLTMRT FHTGGAASLG ITVSDIKVKT AGKIKFKNIR
TVTNKEGQEI VISRAGEIIV SDTMGRVREQ HKIPMGAVVP LASGKAVEIG DVIATWDPHA
QPLITDVAGK VVLEDVIDGI TSKHTYDDLT GQQTIEITSI SQRTTSKNLK PVVKIVDEKG
AELKSIPLAV GAVLNVADDS ILEVGDIVAK IPLEGSKNKD ITGGLPRVAE LFEARRPKDA
AILSPCDGMV RLGNRDTKEK QRIEIIDKNG HIVEEILLPK SRHLVVFDGE QVSRGDVLAD
GPTDPHDLLK YKGLEEFADY ILIEAQSVYR MQGVVINDKH IETIVRQMLR KAVILDEGDS
KFVKDESIEL VRILEENDKL RKQGKKEVEY ELVLMGITRS SLSTESFLSA ASFQETTRVL
TEASINSQID NLRGLKENVL IGRLIPAGTG LAVRKESAKI EKMREELGVE DNMVFTDLSS
FNPEEISFDS IQSQKEDKDI NEDIEESLRN ALESLDF
