ID   ATSE4_PSEAE             Reviewed;         186 AA.
AC   Q9HT95;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Acetyltransferase PA5475 {ECO:0000303|PubMed:23184347};
DE            EC=2.3.1.-;
DE   AltName: Full=GCN5-related N-acetyltransferase {ECO:0000303|PubMed:23184347};
DE            Short=GNAT {ECO:0000303|PubMed:23184347};
GN   OrderedLocusNames=PA5475;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND SUBSTRATE SPECIFICITY.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=23184347; DOI=10.1002/pro.2199;
RA   Kuhn M.L., Majorek K.A., Minor W., Anderson W.F.;
RT   "Broad-substrate screen as a tool to identify substrates for bacterial
RT   Gcn5-related N-acetyltransferases with unknown substrate specificity.";
RL   Protein Sci. 22:222-230(2013).
CC   -!- FUNCTION: Catalyzes the transfer of an acetyl group from acetyl
CC       coenzyme A (AcCoA) to an acceptor substrate and releases both CoA and
CC       the acetylated product. It prefers the antibiotic chloramphenicol.
CC       {ECO:0000269|PubMed:23184347}.
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DR   EMBL; AE004091; AAG08860.1; -; Genomic_DNA.
DR   PIR; E82961; E82961.
DR   RefSeq; NP_254162.1; NC_002516.2.
DR   RefSeq; WP_003096934.1; NZ_QZGE01000012.1.
DR   AlphaFoldDB; Q9HT95; -.
DR   SMR; Q9HT95; -.
DR   STRING; 287.DR97_2854; -.
DR   PaxDb; Q9HT95; -.
DR   PRIDE; Q9HT95; -.
DR   DNASU; 878199; -.
DR   EnsemblBacteria; AAG08860; AAG08860; PA5475.
DR   GeneID; 878199; -.
DR   KEGG; pae:PA5475; -.
DR   PATRIC; fig|208964.12.peg.5739; -.
DR   PseudoCAP; PA5475; -.
DR   HOGENOM; CLU_105788_0_0_6; -.
DR   InParanoid; Q9HT95; -.
DR   OMA; ECECAVT; -.
DR   PhylomeDB; Q9HT95; -.
DR   BioCyc; PAER208964:G1FZ6-5603-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   4: Predicted;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..186
FT                   /note="Acetyltransferase PA5475"
FT                   /id="PRO_0000433350"
FT   DOMAIN          31..186
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT   BINDING         117..119
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT   BINDING         125
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT   BINDING         156
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT   BINDING         161..163
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
SQ   SEQUENCE   186 AA;  21001 MW;  1F917D4570428CF8 CRC64;
     MSNVQNASRS SAFAAVEGDH WVESLDDGRH VLIRPLREED RERERQFINR LSPATRHFRF
     LGEIKEASPA LLDQLMDIDY QQSMAFVALV HEDGELREVG ISRYAACCEE GQCECAVTIA
     DDYQGLGLDA VLMRHLIDVA RRNGFRQMYS VDSAANRAMR DLCCALGFVG QRDPDDSTQV
     IHRLAL