ATSE_AGRFC
ID ATSE_AGRFC Reviewed; 137 AA.
AC Q7CXI0;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Acetyltransferase Atu2258 {ECO:0000303|PubMed:23184347};
DE EC=2.3.1.-;
DE AltName: Full=GCN5-related N-acetyltransferase {ECO:0000303|PubMed:23184347};
DE Short=GNAT {ECO:0000303|PubMed:23184347};
GN OrderedLocusNames=Atu2258;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3]
RP FUNCTION, AND SUBSTRATE SPECIFICITY.
RC STRAIN=C58 / ATCC 33970;
RX PubMed=23184347; DOI=10.1002/pro.2199;
RA Kuhn M.L., Majorek K.A., Minor W., Anderson W.F.;
RT "Broad-substrate screen as a tool to identify substrates for bacterial
RT Gcn5-related N-acetyltransferases with unknown substrate specificity.";
RL Protein Sci. 22:222-230(2013).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
RG Midwest center for structural genomics (MCSG);
RT "Crystal structure of putative acetyltransferase from Agrobacterium
RT tumefaciens.";
RL Submitted (FEB-2006) to the PDB data bank.
CC -!- FUNCTION: Catalyzes the transfer of an acetyl group from acetyl
CC coenzyme A (AcCoA) to an acceptor substrate and releases both CoA and
CC the acetylated product. It prefers glucosamine 6-phosphate or dopamine.
CC It can also use the thialysine, N(8)-acetylspermidine, chloramphenicol,
CC puromycin, polymyxin B, and 4-aminobutyrate ethyl ester.
CC {ECO:0000269|PubMed:23184347}.
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DR EMBL; AE007869; AAK87999.2; -; Genomic_DNA.
DR RefSeq; NP_355214.2; NC_003062.2.
DR RefSeq; WP_010972179.1; NC_003062.2.
DR PDB; 2G3A; X-ray; 1.90 A; A=1-137.
DR PDBsum; 2G3A; -.
DR AlphaFoldDB; Q7CXI0; -.
DR SMR; Q7CXI0; -.
DR STRING; 176299.Atu2258; -.
DR EnsemblBacteria; AAK87999; AAK87999; Atu2258.
DR KEGG; atu:Atu2258; -.
DR PATRIC; fig|176299.10.peg.2268; -.
DR eggNOG; COG0454; Bacteria.
DR HOGENOM; CLU_115862_2_0_5; -.
DR OMA; TWDFQAP; -.
DR PhylomeDB; Q7CXI0; -.
DR BioCyc; AGRO:ATU2258-MON; -.
DR EvolutionaryTrace; Q7CXI0; -.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR Pfam; PF00583; Acetyltransf_1; 1.
DR SUPFAM; SSF55729; SSF55729; 1.
DR PROSITE; PS51186; GNAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..137
FT /note="Acetyltransferase Atu2258"
FT /id="PRO_0000433351"
FT DOMAIN 1..137
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT BINDING 66..68
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT BINDING 74
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT BINDING 108..110
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250|UniProtKB:Q9I0Q8"
FT HELIX 11..29
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 35..42
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 48..57
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 60..67
FT /evidence="ECO:0007829|PDB:2G3A"
FT HELIX 70..72
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:2G3A"
FT HELIX 77..91
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 96..102
FT /evidence="ECO:0007829|PDB:2G3A"
FT HELIX 104..113
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 116..121
FT /evidence="ECO:0007829|PDB:2G3A"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:2G3A"
SQ SEQUENCE 137 AA; 15202 MW; 7E906F947A5DA58E CRC64;
MNFVLSDVAD AEAEKAIRDP LVAYNLARFG ESDKRDLNIT IRNDDNSVTG GLVGHTARGW
LYVQLLFVPE AMRGQGIAPK LLAMAEEEAR KRGCMGAYID TMNPDALRTY ERYGFTKIGS
LGPLSSGQSI TWLEKRF
