RPOC_LEUMM
ID RPOC_LEUMM Reviewed; 1220 AA.
AC P94892; Q03V61;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; OrderedLocusNames=LEUM_1824;
OS Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / DSM
OS 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523 / NBRC 100496 / NCIMB
OS 8023 / NCTC 12954 / NRRL B-1118 / 37Y).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Leuconostoc.
OX NCBI_TaxID=203120;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-1056.
RX PubMed=8863429; DOI=10.1099/00207713-46-4-1004;
RA Morse R., Collins M.D., O'Hanlon K., Wallbanks S., Richardson P.T.;
RT "Analysis of the beta' subunit of DNA-dependent RNA polymerase does not
RT support the hypothesis inferred from 16S rRNA analysis that Oenococcus oeni
RT (formerly Leuconostoc oenos) is a tachytelic (fast-evolving) bacterium.";
RL Int. J. Syst. Bacteriol. 46:1004-1009(1996).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Zn(2+) ion per subunit; 2 are expected compared to other
CC organisms. {ECO:0000305};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000414; ABJ62911.1; -; Genomic_DNA.
DR EMBL; X95810; CAA65077.1; -; Genomic_DNA.
DR RefSeq; WP_011680407.1; NC_008531.1.
DR AlphaFoldDB; P94892; -.
DR SMR; P94892; -.
DR STRING; 203120.LEUM_1824; -.
DR PRIDE; P94892; -.
DR EnsemblBacteria; ABJ62911; ABJ62911; LEUM_1824.
DR GeneID; 61177761; -.
DR KEGG; lme:LEUM_1824; -.
DR eggNOG; COG0086; Bacteria.
DR HOGENOM; CLU_000524_3_1_9; -.
DR OMA; YRNIRVE; -.
DR OrthoDB; 4421at2; -.
DR Proteomes; UP000000362; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Transcription; Transferase; Zinc.
FT CHAIN 1..1220
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000067752"
FT REGION 1197..1220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1203..1220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 63
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 76
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 79
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 450
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 452
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 454
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT CONFLICT 827
FT /note="V -> A (in Ref. 2; CAA65077)"
FT /evidence="ECO:0000305"
FT CONFLICT 842
FT /note="D -> V (in Ref. 2; CAA65077)"
FT /evidence="ECO:0000305"
FT CONFLICT 863
FT /note="V -> C (in Ref. 2; CAA65077)"
FT /evidence="ECO:0000305"
FT CONFLICT 988
FT /note="A -> E (in Ref. 2; CAA65077)"
FT /evidence="ECO:0000305"
FT CONFLICT 991
FT /note="T -> A (in Ref. 2; CAA65077)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1220 AA; 135789 MW; D42BC98F05B9EBAD CRC64;
MAIDVNKFES MQIALASPDK IRSWSYGEVK KPETINYRTL KPEKDGLFDE RIFGPTKDYE
CACGKYKRIR YKGIVCDRCG VEVTSSKVRR ERMGHIELAA PVTHIWYFKG IPSRMGLVLD
MSPRSLEEII YFASYVVIEP GDAPVEKKQM MTEREYRQLK KEYGAGFKAG MGAEAIKELL
ANVDLATEAD ELKRELQEAT GQKRVRAVRR LDIIEAFLQS DNKPEWMVME VIPVIPPDLR
PMVQLEGGRF ATSDLNDLYR RVINRNNRLK RLLDLNAPGI IVQNEKRMLQ EAVDALIDNG
RRGRPVAGPG NRPLKSLSHM LKGKQGRFRQ NLLGKRVDYS GRSVIDVGPF LKMNQMGLPV
PMAIELFRPF IMKELTTRKL AGNVKSAKRK IDKADGDVMD VLEDVIKEHP VLLNRAPTLH
RLGIQAFEPV LVSGKAMRLH PLVTEAYNAD FDGDQMAIHV PLSDEAQAEA RLLMLAAGHI
LAPKDGKPIV APSQDMVIGN YYLTTEEAGR EGEGMIFSSV DEARIAFASK VVHYHTRVGI
QTSSFPSEKP FTDEQRSKIM ITSVGKLFFN EILPSDFPYI NEPSEDNFKR VDDSFFIDAG
ENIHDYLADT SIVNPFKKGF LSDIIAEVYK RYKVTETSLL LDRMKDLGYD KSTESGLTVS
MTDVLELEEK PAILEDAHSQ VATVTKQFRR GLITDSERYQ RVTEIWTKAK DIIQDKLIES
FEPTNPIFMM QDSGARGNIS NFVQLAGMRG LMAGPGGKII ELPVTANFRE GLTVMEMFIS
THGARKGMSD TALKTANSGY LTRRLVDVAQ DVIVREFDND SDRGVAVQAI MDGTSVVEPL
YDRILGRYAM KSVFDPETDE KIVSRNEMID EDVAKKIVNA GIKEVTIRSV FTSTTEHGVS
VLDYGRNLAS GEEVEVGEAV GTVAAQSIGE PGTQLTMRNF HTGGVAGGND ITQGLPRVQE
IVEARIPKGR AEISEVTGTI TVIEENPADR TKSVTIEGET DTRTYTLPLT TRMRFGEGDE
IKRGEAINEG PIDPKELLAV TDTLTTESYM LTEIQKVYRL QGIEVSDKHI EVMIRQMLRK
IRVMDPGQTD LLPGTLMDIA DFKRANEPAL FEGLVPATAR PVLLGITKAA LETNSFLSAA
SFQETTRVLT DAAIRGKNDP LVGLKENVII GKIIPAGTGM AEYRKIKSKV VGDVIAQPES
ESEEASDIPK LDDVAKTFDN
