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RPOC_MYCGE
ID   RPOC_MYCGE              Reviewed;        1292 AA.
AC   P47582; Q49292; Q49454; Q49483; Q49486;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2018, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE   AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN   Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; OrderedLocusNames=MG340;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-142 AND 1029-1148.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-506.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=1945886; DOI=10.1093/nar/19.21.6027;
RA   Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A random sequencing approach for placing markers on the physical map of
RT   Mycoplasma genitalium.";
RL   Nucleic Acids Res. 19:6027-6031(1991).
RN   [4]
RP   SEQUENCE REVISION TO 818, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=16407165; DOI=10.1073/pnas.0510013103;
RA   Glass J.I., Assad-Garcia N., Alperovich N., Yooseph S., Lewis M.R.,
RA   Maruf M., Hutchison C.A. III, Smith H.O., Venter J.C.;
RT   "Essential genes of a minimal bacterium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:425-430(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01322};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- DISRUPTION PHENOTYPE: Probably essential, it was not disrupted in a
CC       global transposon mutagenesis study. {ECO:0000269|PubMed:16407165}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01322}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA43746.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; L43967; AAC71565.2; -; Genomic_DNA.
DR   EMBL; U01797; AAD12323.1; -; Genomic_DNA.
DR   EMBL; U02169; AAD12451.1; -; Genomic_DNA.
DR   EMBL; X61528; CAB98132.1; -; Genomic_DNA.
DR   EMBL; X61534; CAA43746.1; ALT_FRAME; Genomic_DNA.
DR   PIR; F64237; F64237.
DR   RefSeq; WP_010869441.1; NC_000908.2.
DR   AlphaFoldDB; P47582; -.
DR   SMR; P47582; -.
DR   STRING; 243273.MG_340; -.
DR   EnsemblBacteria; AAC71565; AAC71565; MG_340.
DR   KEGG; mge:MG_340; -.
DR   eggNOG; COG0086; Bacteria.
DR   HOGENOM; CLU_000524_3_1_14; -.
DR   OMA; YRNIRVE; -.
DR   OrthoDB; 4421at2; -.
DR   BioCyc; MGEN243273:G1GJ2-427-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   Gene3D; 4.10.860.120; -; 1.
DR   HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
DR   TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW   Nucleotidyltransferase; Reference proteome; Transcription; Transferase;
KW   Zinc.
FT   CHAIN           1..1292
FT                   /note="DNA-directed RNA polymerase subunit beta'"
FT                   /id="PRO_0000067759"
FT   BINDING         70
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         72
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         85
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         88
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         532
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         534
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         536
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         911
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         987
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         994
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   BINDING         997
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT   CONFLICT        33..36
FT                   /note="WSEG -> LIWR (in Ref. 2; AAD12323)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        338..344
FT                   /note="NDRLRRI -> KWPIKKD (in Ref. 3; CAA43746)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1029..1037
FT                   /note="LTMRTFHTG -> MDNAYFPYW (in Ref. 2; AAD12451)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1292 AA;  145705 MW;  AF5939913B87DF4D CRC64;
     MTTTRRNKRN NKLYKNIKAI KLSIASNDTI LNWSEGEVTK AETINYKSLK PEPGGLFDEA
     IFGPVKDYEC ACGKFKKIKY RGVRCDRCGV WVTESIVRRE RMGHIALVSP VAHIWMSKEL
     PSPSKISLVL NISYKEVEQV LYFVNYIVLD TGKIKDDKIM PFKFKEVLDL TGKGSLSTRQ
     KMRRVIGYIF RNLIKSKSSE DYRKGKIFYE SLKNSSLPFS LNDAFNYIKK YTGFRVGIGA
     EAILELLNKI DLNLEFSRLN DALRKAKKDS VEDAKVKKIL RQLETISWFR NSKLHPKNMI
     LHTVPVIPPD IRPIIQLDGA KFTTSDINNF YRRVIIRNDR LRRILEDGTV PSIVVNNEKR
     LLQESVDALF DNSSRHKPSL SKDKRSLKSL TDRLKGKQGL FRHNLLGKRV DYSGRSVIVV
     GPELKMYEVG IPALMILKLF KPFIIHGLIN KFDENGNEIR PIAASIRQAE DMIKNQDDLI
     WGIVYDVIKD RPVLLNRAPT LHRLGIQAFE PRIVDGKAIR LHPLVTTAFN ADFDGDQMAV
     HVPLSENAVN EARAVLLASK HILGLKDGRP IVTPTQDMVL GNYYLTTERK GQLGEGIIFS
     TVYEARAAYE SQKVHLHAIV GISTKAFPNK KFACQGTLIT TVGKIIFNDV LGNNVPYIND
     GEFDENACPE KFIVKQGEDV RQSILKHQII PAFSKKVISK LIDLLYLLLE FKDLPKTLDN
     IKALGFKYST FSSTTVSVFD IPKYTNKQNY FDSADQQVLK YKQFYNKGLL TDDERYKRVV
     KLWNNVKEKV SDEIQNLIKQ EQYRDNSIVV MADSGARCNI SNFTQLFGMR GLMSKSFNYE
     RNNQSKIIKD TIEVPIKHSF FEGLTINEYF NSSYGARKGM TDTAMKTAKS GYMTRKLVDA
     THELIINHDD CGTRKGIVVE AIVETKTKSL IESLFDRIVN RYSITPIVDP ETQKTIVEAN
     SLITTQLAKQ ICATSIKEVL VRSVIYCERE NGICQYCFGI DLSTGKLVEL GTAVGVIAAQ
     SIGEPGTQLT MRTFHTGGVS TENNLAQGFE RLKQIFEVVT PKDFEKAVIS EVKGTVKSIT
     TVQNAQEVVI KSNVDERIYT IPFSAQIRVH VGDQVSPGSK ITEGSVDIKQ LLRIAGIQRV
     RQYMIVEIQK VYRIQGIDIA DKYVEIIIRQ LTNLLQVTDA GNSNLFVGQL VHSHYLNELN
     KSLLLAGKMP VIAINQVFGI DEAASKSNSF LSAASFQDTK KILTDAAVKN QVDYLLGLKE
     NVIIGGKIPA GTGFLTDEEL TFLGSKTVAE EY
 
 
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