ID   ATSI_AMACA              Reviewed;          69 AA.
AC   P80211;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Trypsin/subtilisin inhibitor;
DE   AltName: Full=ATSI;
OS   Amaranthus caudatus (Love-lies-bleeding) (Inca-wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Amaranthaceae; Amaranthus.
OX   NCBI_TaxID=3567;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. CAC 064; TISSUE=Seed;
RX   PubMed=8305477; DOI=10.1016/0167-4838(94)90034-5;
RA   Hejgaard J., Dam J., Pedersen L.C., Bjoern S.E.;
RT   "Primary structure and specificity of the major serine proteinase inhibitor
RT   of amaranth (Amaranthus caudatus L.) seeds.";
RL   Biochim. Biophys. Acta 1204:68-74(1994).
CC   -!- FUNCTION: Inhibitor of trypsin, chymotrypsin, subtilisin, etc.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; S40496; S40496.
DR   PDB; 7AL8; X-ray; 2.85 A; C/F/I/L/O/R/V/X=1-69.
DR   PDBsum; 7AL8; -.
DR   AlphaFoldDB; P80211; -.
DR   SMR; P80211; -.
DR   MEROPS; I13.009; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Serine protease inhibitor.
FT   CHAIN           1..69
FT                   /note="Trypsin/subtilisin inhibitor"
FT                   /id="PRO_0000217641"
FT   SITE            45..46
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        4..49
FT                   /evidence="ECO:0000250"
FT   HELIX           11..13
FT                   /evidence="ECO:0007829|PDB:7AL8"
FT   HELIX           18..28
FT                   /evidence="ECO:0007829|PDB:7AL8"
FT   STRAND          33..38
FT                   /evidence="ECO:0007829|PDB:7AL8"
FT   STRAND          51..56
FT                   /evidence="ECO:0007829|PDB:7AL8"
FT   STRAND          60..62
FT                   /evidence="ECO:0007829|PDB:7AL8"
SQ   SEQUENCE   69 AA;  7889 MW;  3000305F2D73943F CRC64;
     ARECPGKQEW PELVGEYGYK AAAIIERENP NVRSIVKHER SGFTKDFRCD RVWVVVDSTG
     VVVRTPRVT