RPOC_OPITP
ID RPOC_OPITP Reviewed; 1384 AA.
AC B1ZPB7;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; OrderedLocusNames=Oter_0231;
OS Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1).
OC Bacteria; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus.
OX NCBI_TaxID=452637;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11246 / JCM 15787 / PB90-1;
RX PubMed=21398538; DOI=10.1128/jb.00228-11;
RA van Passel M.W., Kant R., Palva A., Copeland A., Lucas S., Lapidus A.,
RA Glavina del Rio T., Pitluck S., Goltsman E., Clum A., Sun H., Schmutz J.,
RA Larimer F.W., Land M.L., Hauser L., Kyrpides N., Mikhailova N.,
RA Richardson P.P., Janssen P.H., de Vos W.M., Smidt H.;
RT "Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an
RT abundant inhabitant of rice paddy soil ecosystems.";
RL J. Bacteriol. 193:2367-2368(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Zn(2+) ion per subunit; 2 are expected compared to other
CC organisms. {ECO:0000305};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
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DR EMBL; CP001032; ACB73522.1; -; Genomic_DNA.
DR RefSeq; WP_012373060.1; NC_010571.1.
DR AlphaFoldDB; B1ZPB7; -.
DR SMR; B1ZPB7; -.
DR STRING; 452637.Oter_0231; -.
DR PRIDE; B1ZPB7; -.
DR EnsemblBacteria; ACB73522; ACB73522; Oter_0231.
DR KEGG; ote:Oter_0231; -.
DR eggNOG; COG0086; Bacteria.
DR HOGENOM; CLU_000524_3_1_0; -.
DR OMA; YRNIRVE; -.
DR OrthoDB; 4421at2; -.
DR Proteomes; UP000007013; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase;
KW Zinc.
FT CHAIN 1..1384
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000353398"
FT BINDING 81
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 83
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 96
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 99
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 472
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 474
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 476
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
SQ SEQUENCE 1384 AA; 152789 MW; 84B0D78CDCB261D7 CRC64;
MSIQPSEVAA SSARTDTREA LGIEESAFDC VSITVASPET IRKWSKGEVK NPETINYRTF
KPEPGGLFCQ KIFGPVRDYE CACGKYKRIK YKDVVCDRCG VEVTIARVRR ERMGHIELAV
PVAHIWFLKS MPSRLGLLLD MTARSLERVI YYENYMVIDP GKTPLEPHQL LTDTEYRQAI
DEYGEDSFVA KMGAEAVRDA LVKTDLEATV AELQEQMRAT KSKQIKKKLS KRLKVIQGFI
HSKSRPEWMV LEVLPVIPPD LRPLVPLEGG RFATSDLNDL YRRVINRNNR LRNLMQLKTP
DVIIHNEKRM LQEAVDALFD NGRHGRPVTG AGNRPLKSLS DMLKGKQGRF RQNLLGKRVD
YSGRSVIVIG PELKLHQCGL PKKMALVLFE PFIIRRLKEL GFVHTVRGAR KMIEKKSPEV
WDILEEVTKG HPVLLNRAPT LHRLSIQAFE PVLIEGEAIR VHPLVCTAYN ADFDGDQMAV
HVPLSLEAIM ECKLLMMATS NIFSPSSGKP ILTPSQDIVL GAYYLTVEPR KKPAKDERVP
LLSGLQEVLY AVADGAMKKH DWVEVPNPDH GRETIFGNKE KKVLRTTVGR VIFNQIWPAG
LGFVNFPVPK SKLGDLILNT HKLTGNQATV ETLDRLKELG FTTAMQAGIS IGIDDMIIPE
AKKDIVAETR KKIAEVEAQF NKGIITEGER KNKVIDLWTG TTDRIAKEVF AKLESNEGRN
EVNPVYIMMD SGARGNKQQV RQLCGTRGLM AKPSGEIIER PILSSFREGL TVLEYFISTH
GARKGLADTA LKTADAGYLT RKLCDVAMDV IIAEDDCGSR DGVWKKAIFE GDDEIVSLRE
RIVGRFSSDD VFNPINPSEI LVGSGELITE EIATRVDELG IERVKVMSPL TSTAQHGIDG
KSYGINPATG KVAKVGDSVG IIAAQSIGEP GTQLTMRTFH IGGVASGGFK TPEIKVRASG
TVRYRGLRLV ETADGGSIVL NKTGTIQIVD AEEKELETYN IVVGSFLHVG DGEQIQKGAI
LAQWDPYNIP VLSEKGGTLA FKDMIPGVTV KRELDESSGR IATVVIEHKE DLNPQIEIRD
PKGKPLAAYS IPVGAQIAVN EGDIIQPGAL LAKTPRQASK TKDITGGLPR VAELFEARRP
KDAAEMSRID GIVSFEGTVR GKRKLVVKND DTAQEEEHLI ATGKHIIVQP GDVVHKGQHL
TEGAADPHEI LEILGPSALY DFLISQVQEV YRLQGVAIND KHIEIIIRQM LRKVRITDPG
DTENFWGEQV DRAQFLAENR RIEEAGGKPA EAEPILLGIT KASLETESFI SAASFQETTR
VLTDASTLGK VDMLKGFKEN VIMGHLIPAG TGLPKYKNLK ITLPFGADLP VEPEQPAPAA
TETA
