RPOC_WEIHE
ID RPOC_WEIHE Reviewed; 1046 AA.
AC P96177;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Flags: Fragment;
GN Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322};
OS Weissella hellenica.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Weissella.
OX NCBI_TaxID=46256;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 51523 / DSM 7378 / CCUG 33494 / LMG 15125 / NBRC 15553 / NCFB
RC 2973 / LV346;
RX PubMed=8863429; DOI=10.1099/00207713-46-4-1004;
RA Morse R., Collins M.D., O'Hanlon K., Wallbanks S., Richardson P.T.;
RT "Analysis of the beta' subunit of DNA-dependent RNA polymerase does not
RT support the hypothesis inferred from 16S rRNA analysis that Oenococcus oeni
RT (formerly Leuconostoc oenos) is a tachytelic (fast-evolving) bacterium.";
RL Int. J. Syst. Bacteriol. 46:1004-1009(1996).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01322, ECO:0000305}.
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DR EMBL; X96470; CAA65322.1; -; Genomic_DNA.
DR AlphaFoldDB; P96177; -.
DR SMR; P96177; -.
DR STRING; 46256.BBIK01000001_gene266; -.
DR eggNOG; COG0086; Bacteria.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Transcription; Transferase; Zinc.
FT CHAIN <1..>1046
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000067831"
FT BINDING 383
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 385
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 387
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 752
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 826
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 833
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 836
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT NON_TER 1
FT NON_TER 1046
SQ SEQUENCE 1046 AA; 117107 MW; D54C62C26A7F1696 CRC64;
RIRYKGIVCD RCGVEVTSAK VRRERMGHIE LAAPVSHIWY FKGIPSRMGL VLDMSPRSLE
EVIYFASYVV TKPGDTSLTE KQLLSEREYR QLKAEYGNAF EAGMGAEAVQ TLLSNVDLEE
EVTELKAQLR EATGQKRVRA VRRLDIIEAF VKSGNKPEWM VMDVIPIIPP DLRPMVQLEG
GRFATSDLND LYRRVINRNN RLKRLLELNA PGIIVQNEKR MLQEAADALV DNGRRGRPVT
GPGNRPLKSL SHMLKGKQGR FRQDLLGKRV DYSGRSVIDV GPFLKMNQMG LPRPMAMELF
RPFIMKELVK RDLAGNIRAA KRKIDRHDED VMDVLEDVIK EHPVLLNRAP TLHRLGIQAF
EPVLVSGKAM RLHPLVTEAY NADFDGDQMA IHVPLSDEAQ AEARLLMLAA GHILAPKDGK
PIVAPSQDMV IGNYYLTTEE AGREGEGMIF KDVNEVRTAY QNKYVHLHTR IGIQTASLSA
EKPFTAEQRS RIMMTSVGKL FFNDILPADF PYLNEPTEAN LHEIDNRFFL EPGEDIKARY
AETPILPPFK KGYLSDIIAE VYKIYKVTET SLLLDRMKDL GYDESTKSGL TVGVADVTDL
KEKPEIIEDA HKQVATVTKQ FRRGLITDSE RYERVIAIWN KAKDDITEKL IEHFEPDNNI
FMMSDSGARG NISNFTQLAG MRGLMAAPNG RIMELPIIAN FREGLSVLEM FFSTHGARKG
MTDTALKTAD SGYMTRRLVD VAQDVIIRED DCGSDRGLDV SAIMNGNEVI ESLYERILGR
YAQKSVFEPQ TGDKLVGHNE MITEDIAKRI IEAGVTTVTI RSAFTCNTEH GVCVRCYGRN
MATGDVVEVG EAVGTVAAQS IGEPGTQLTM RTFHTGGVAG NDITQGLPRV QEIFEARNPK
GRAMITEVTG EVTTIEENPA DRTKEVTIQG ETDTRTYTLP MSSRMRVGEG DHIHRGETLN
EGSADPKEII QVRDTLATEN YIVLEVQKVY RMQGVEISDK HIEVMARQML RKVRVMDPGE
TDLLPGTLMD IAQFRDANEG TLLKGG
