RPOC_XANOP
ID RPOC_XANOP Reviewed; 1405 AA.
AC B2SQQ2;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01322};
GN Name=rpoC {ECO:0000255|HAMAP-Rule:MF_01322}; OrderedLocusNames=PXO_04529;
OS Xanthomonas oryzae pv. oryzae (strain PXO99A).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=360094;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PXO99A;
RX PubMed=18452608; DOI=10.1186/1471-2164-9-204;
RA Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D.,
RA Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R.,
RA Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A.,
RA Patil P.B., Ishihara H., Meyer D.F., Szurek B., Verdier V., Koebnik R.,
RA Dow J.M., Ryan R.P., Hirata H., Tsuyumu S., Won Lee S., Seo Y.-S.,
RA Sriariyanum M., Ronald P.C., Sonti R.V., Van Sluys M.-A., Leach J.E.,
RA White F.F., Bogdanove A.J.;
RT "Genome sequence and rapid evolution of the rice pathogen Xanthomonas
RT oryzae pv. oryzae PXO99A.";
RL BMC Genomics 9:204-204(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01322};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01322};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01322};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01322}.
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DR EMBL; CP000967; ACD57879.1; -; Genomic_DNA.
DR RefSeq; WP_011260033.1; NC_010717.2.
DR PDB; 6J9E; EM; 3.41 A; D=1-1405.
DR PDB; 6J9F; EM; 3.95 A; D=1-1405.
DR PDBsum; 6J9E; -.
DR PDBsum; 6J9F; -.
DR AlphaFoldDB; B2SQQ2; -.
DR SMR; B2SQQ2; -.
DR STRING; 360094.PXO_04529; -.
DR EnsemblBacteria; ACD57879; ACD57879; PXO_04529.
DR KEGG; xop:PXO_04529; -.
DR eggNOG; COG0086; Bacteria.
DR HOGENOM; CLU_000524_3_1_6; -.
DR OMA; YRNIRVE; -.
DR OrthoDB; 4421at2; -.
DR Proteomes; UP000001740; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR012754; DNA-dir_RpoC_beta_prime_bact.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Transcription; Transferase; Zinc.
FT CHAIN 1..1405
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000353459"
FT REGION 1375..1405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 72
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 85
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 88
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 460
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 462
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 464
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 815
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 890
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 897
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT BINDING 900
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01322"
FT HELIX 6..8
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 20..24
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 27..33
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 86..88
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 95..98
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 104..112
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 114..117
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 118..121
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 123..127
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 132..139
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 142..146
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 162..172
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 182..190
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 196..205
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 212..229
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 234..236
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 237..243
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 247..249
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 265..284
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 289..307
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 311..313
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 327..331
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 332..336
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 339..343
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 344..357
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 359..361
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 363..369
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 370..376
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 378..388
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 394..403
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 406..415
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 416..418
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 421..427
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 431..433
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 434..449
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 451..453
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 454..457
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 461..463
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 465..469
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 474..483
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 486..488
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 493..499
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 504..514
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 530..538
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 547..556
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 565..574
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 575..580
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 581..583
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 586..588
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 592..595
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 599..612
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 616..634
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 635..637
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 642..645
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 649..670
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 676..703
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 707..710
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 713..716
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 722..729
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 730..732
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 735..738
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 739..741
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 764..766
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 770..804
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 821..823
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 829..834
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 836..839
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 843..847
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 868..877
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 882..884
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 887..889
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 893..896
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 898..901
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 905..910
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 919..925
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 928..930
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 980..987
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 989..991
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 996..999
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1018..1020
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1140..1148
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1153..1155
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1163..1165
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1172..1180
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1182..1184
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1187..1192
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1210..1212
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1218..1224
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1228..1245
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1251..1261
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1264..1269
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1271..1273
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1278..1280
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1281..1293
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1303..1305
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1310..1313
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1320..1323
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1325..1327
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1329..1339
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1349..1354
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1359..1361
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1364..1370
FT /evidence="ECO:0007829|PDB:6J9E"
SQ SEQUENCE 1405 AA; 155232 MW; 16AAA34E5554208F CRC64;
MKDLLNLFNQ QRQTLDFDAI KIALASPDLI RSWSYGEVKK PETINYRTFK PERDGLFCAA
IFGPIKDYEC LCGKYKRMKH RGVVCEKCGT EVTLAKVRRE RMGHIDLASP VAHIWFLKSL
PSRIGLMLDM TLRDIERVLY FEAYVVTEPG LTPLERRQLL TEEQYLTARQ EYNDDFDAAM
GAEAVYELLR TIDLQSEMTR LREEIASTGS ETKLKRLTKR IKLIEAFLES GNRPEWMVMT
VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLR RLLELNAPDI IVRNEKRMLQ
ESVDALLDNG RRGRAITGTN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY
LKLHQCGLPK KMALELFKPF VFAKLQRRGL ATTIKAAKKL VEREEAEVWD ILEEVIREHP
VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCTAFNAD FDGDQMAVHV PLSLEAQLEA
RALMMSTNNI LSPANGEPII VPSQDVVLGL YYMSRALENK KGEGMVFANT SEVKRAYDNR
VVELHAKVKV RITQVDVDAV DGKRTSGTSI VDTTVGRALL SEILPEGLPF QLANTEMTKK
NISRLINSSY RLLGLKDTVV FADKLMYTGY AYATRAGVSI GIDDMLIPDE KKGILTEAEA
EVLEIQEQYQ SGLVTAGERY NKVVDIWSRT SERIAKAMMD TIGTEKVENA KGETIDQKSM
NSLYIMADSG ARGSQAQIRQ LAGMRGLMAR PDGSIIETPI KANFREGLNV QEYFNSTHGA
RKGLADTALK TANSGYLTRR LVDVAQDVVI TEIDCGTTEG LIMTPIVEGG DVVEPLKERV
LGRVVAEDVY LPGNDEEPIV TRNTLLDEAW VAKLEDASVQ SVKVRSTISC ESSFGVCARC
YGRDLARGHQ VNIGEAVGVI AAQSIGEPGT QLTMRTFHIG GAASRAAAVD NITVKTTGSV
KFNNLKSVAH ASGSLVAVSR SGELSVLDGH GRERERYKLP YGATITAKDG DAVKAGQSVA
NWDPHNHPIV SEVAGFIRFI DFVDGVTVIE KTDELTGLAS REITDPKRRG AHAKELRPIV
RIVDGKGNDL TIPNTDLPAQ YLLPPRSIVN LQDGAAVGVG DVVAKIPQEA SKTRDITGGL
PRVADLFEAR KPKDPAILAE RSGIISFGKD TKGKQRLIIK DTDGSEHEEL IPKYRQIIVF
EGEHVTKGET VVDGEPSPQD ILRLLGVEPL AAYLVKEIQD VYRLQGVKIN DKHIEVITRQ
MLRKVEIVDQ GNSKFLNGEQ VERQRVIEEN ARLVKRNELP AKYDPVLLGI TKASLATESF
ISAASFQETT RVLTEAAVRG TRDNLRGLKE NVIVGRLIPA GTGLAYHAGR RKASGLTDSE
METLSGKPAG AEPVAALADA GADEE
