RPOD_BUCAP
ID RPOD_BUCAP Reviewed; 617 AA.
AC P32001;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; OrderedLocusNames=BUsg_052;
OS Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=198804;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1398077; DOI=10.1016/0378-1119(92)90074-y;
RA Lai C.-Y., Baumann P.;
RT "Sequence analysis of a DNA fragment from Buchnera aphidicola (an
RT endosymbiont of aphids) containing genes homologous to dnaG, rpoD, cysE,
RT and secB.";
RL Gene 119:113-118(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sg;
RX PubMed=12089438; DOI=10.1126/science.1071278;
RA Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT "50 million years of genomic stasis in endosymbiotic bacteria.";
RL Science 296:2376-2379(2002).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; M90644; AAA73234.1; -; Genomic_DNA.
DR EMBL; AE013218; AAM67623.1; -; Genomic_DNA.
DR PIR; JC1291; RNJV7A.
DR RefSeq; WP_011053589.1; NC_004061.1.
DR AlphaFoldDB; P32001; -.
DR SMR; P32001; -.
DR STRING; 198804.BUsg_052; -.
DR PRIDE; P32001; -.
DR EnsemblBacteria; AAM67623; AAM67623; BUsg_052.
DR KEGG; bas:BUsg_052; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_7_0_6; -.
DR OMA; RDAKKEM; -.
DR OrthoDB; 1565734at2; -.
DR Proteomes; UP000000416; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04546; Sigma70_ner; 1.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..617
FT /note="RNA polymerase sigma factor RpoD"
FT /id="PRO_0000093878"
FT DNA_BIND 577..596
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 192..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..453
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 462..538
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 551..604
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 407..410
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 195..217
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 617 AA; 71470 MW; 95DC5864D463AD67 CRC64;
MEHNPKSQLK LLVTHGKEQG YLTYAEVNDH LPEEIIDSEQ IDDIIQMIND MGIQVVEEAP
DADDLILNEI NTDTDEDAVE AATQVLSSVE SELGRTTDPV RMYMREMGTV ELLTREGEID
IAKRIEEGIN QVQSSVSEYP EAITYLLEQY DRIKTGQIRL SDIITGFVDP NAEEIFSHST
SAIHIGSEIL DNITNDSNEN EDENEDENED EDENSIDPEL ANEKFRELRK QYTNTSNTIK
NKNRNHQDSL LEIYNLSEIF KQFRLVPKQF DHLVNNMRNM MNRVRKQERK IMKLCIEECK
IPKKIFLKIF SGKETDQNWF KKEQSSNQSW SKKLEEIKEK VFSSMKKLKQ IEKETGLTIE
QVKDINKRMS IGEAKAKRAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV
DKFEYRRGYK FSTYATWWIR QAITRSIADQ ARTIRIPVHM IETINKLNRI SRQMLQEIGR
EPTPEELSEK MLIPEDKIRK VLKIAKEPIS METPIGDDDD SHLGDFIEDT TLELPLDSAT
SESLRSATHD VLSGLTAREA KVLRMRFGID MNTDHTLEEV GKQFDVTRER IRQIEAKALR
KLRHPSRSEV LRSFLDD
