ID   RPOD_BUCBP              Reviewed;         609 AA.
AC   Q89B10;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2003, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; OrderedLocusNames=bbp_052;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; AE016826; AAO26791.1; -; Genomic_DNA.
DR   RefSeq; WP_011091192.1; NC_004545.1.
DR   AlphaFoldDB; Q89B10; -.
DR   SMR; Q89B10; -.
DR   STRING; 224915.bbp_052; -.
DR   EnsemblBacteria; AAO26791; AAO26791; bbp_052.
DR   GeneID; 56470596; -.
DR   KEGG; bab:bbp_052; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_7_0_6; -.
DR   OMA; RDAKKEM; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04546; Sigma70_ner; 1.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..609
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093879"
FT   DNA_BIND        569..588
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          375..445
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          454..530
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          543..596
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           399..402
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   609 AA;  70625 MW;  2BC6E11F8D1FAF9B CRC64;
     MEQNPQSQLK LLVTYGKEQG YLTYSEINDH LSDNIINSDQ IEDIIQMIND MGIQVVEKAP
     DSDDLILHEI KRNNETDEDI VEATAQVLST VESELGRTTD PVRMYMREMG TVELLTREGE
     IDIAKRIEDG INQVQCSVAE YPEAITHLLE QYNRVELGEL RLSELIHGFV DPNAEAVHTS
     ITNHINTNSE EHHHNDNTEE NNDDDHLVDP ELAREKFIAL KNQYHITNYA IKTKNRNHQD
     TLIEINNLSE IFKQFRLVPK QFDHLVINMR KMMQKIRIQE RNIAKLCIEY GNIPKKALLN
     FFSLQKTNQI WSKIVQNIKK PWIDKFNTIK PDIQMILKKL QKVEEETGLT IHQVKNINKR
     ISLGEAKAKR AKKEMVEANL RLVISIAKKY TNRGLQFLDL IQEGNIGLMK AVDKFEYRRG
     YKFSTYATWW IRQAITRSIA DQARTIRIPV HMIETINKLN RISRQILQEI GREPTPEELS
     ERMLIPEDKI RKVLKIAKEP ISMETPIGED DDSHLGDFLE DTNIELPLDS ATSASLRSAT
     KNVLSGLTTR EAKVLRMRFG IDMNTDHTLE EVGKQFDVTR ERIRQIEAKA LRKLRHPSRS
     EILRSFLDD