ID   RPOD_CAUVC              Reviewed;         652 AA.
AC   P52324;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; OrderedLocusNames=CC_3047;
OS   Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=190650;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19089 / CB15;
RX   PubMed=7543475; DOI=10.1128/jb.177.15.4372-4376.1995;
RA   Malakooti J., Wang S.P., Ely B.;
RT   "A consensus promoter sequence for Caulobacter crescentus genes involved in
RT   biosynthetic and housekeeping functions.";
RL   J. Bacteriol. 177:4372-4376(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19089 / CB15;
RX   PubMed=11259647; DOI=10.1073/pnas.061029298;
RA   Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA   Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA   Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA   Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA   Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA   Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT   "Complete genome sequence of Caulobacter crescentus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; U35138; AAA82054.1; -; Genomic_DNA.
DR   EMBL; AE005673; AAK25009.1; -; Genomic_DNA.
DR   PIR; I40676; I40676.
DR   RefSeq; NP_421841.1; NC_002696.2.
DR   RefSeq; WP_010920883.1; NC_002696.2.
DR   AlphaFoldDB; P52324; -.
DR   SMR; P52324; -.
DR   STRING; 190650.CC_3047; -.
DR   PRIDE; P52324; -.
DR   EnsemblBacteria; AAK25009; AAK25009; CC_3047.
DR   KEGG; ccr:CC_3047; -.
DR   PATRIC; fig|190650.5.peg.3051; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_7_2_5; -.
DR   OMA; IYAYKDA; -.
DR   BioCyc; CAULO:CC3047-MON; -.
DR   Proteomes; UP000001816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04546; Sigma70_ner; 1.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..652
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093880"
FT   DNA_BIND        612..631
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          194..236
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          418..488
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          497..573
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          586..639
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           442..445
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   COMPBIAS        215..230
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   652 AA;  72675 MW;  FF72B71CD8C96B8B CRC64;
     MSNNSSAETE APETTGGDGP LLDLTDAGVK KFIKQAKARG YVTMDELNKV LPSEEVSPDA
     IEDTLAMLSE MGVNVVEAEE DAENAEGGEV ATRDENTTVI ASDKPAAYDR TDDPVRMYLR
     EMGSVELLSR EGEIAIAKRI EAGRDTMIRG LCESALTFEA IMVWREELGT GRILLREVID
     LEGTYAAING VAAQPAAEDD EGPAEPVDDE AGEAKAEGAE GEDEDDFDDG AGPTVSAMEG
     ELREGVMAIL DAIASEFEAF RKLQDKLVGS RLKGEDLSDA DRKAYEGLSA TIIQHLKTLK
     LNNNRIEALV EQLYAINKRL IGLEGRLLRL ADSYGISRGE FLKAYFGSEL NPTWSEQVKA
     MGVRWTKFVE NDSQSVTDIR SEIAALATET GVPIDDYRRI VQTVQKGERE ARQAKKEMVE
     ANLRLVISIA KKYTNRGLQF LDLIQEGNIG LMKAVDKFEY RRGYKFSTYA TWWIRQAITR
     SIADQARTIR IPVHMIETIN KIVRTSRQML HEIGREPTPE ELAEKLAMPL EKVRKVLKIA
     KEPISLETPI GDEEDSHLGD FIEDKNAILP IDAAIQSNLR ETTTRVLASL TPREERVLRM
     RFGIGMNTDH TLEEVGQQFS VTRERIRQIE AKALRKLKHP SRSRKLRSFL DS