RPOD_HELPY
ID RPOD_HELPY Reviewed; 671 AA.
AC P55993;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; OrderedLocusNames=HP_0088;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- INTERACTION:
CC P55993; O34754: HP_1535; NbExp=4; IntAct=EBI-7494721, EBI-7707586;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; AE000511; AAD07156.1; -; Genomic_DNA.
DR PIR; H64530; H64530.
DR RefSeq; NP_206888.1; NC_000915.1.
DR RefSeq; WP_000725929.1; NC_018939.1.
DR AlphaFoldDB; P55993; -.
DR SMR; P55993; -.
DR DIP; DIP-3055N; -.
DR IntAct; P55993; 14.
DR MINT; P55993; -.
DR STRING; 85962.C694_00430; -.
DR PaxDb; P55993; -.
DR EnsemblBacteria; AAD07156; AAD07156; HP_0088.
DR KEGG; hpy:HP_0088; -.
DR PATRIC; fig|85962.47.peg.94; -.
DR eggNOG; COG0568; Bacteria.
DR OMA; IYAYKDA; -.
DR PhylomeDB; P55993; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..671
FT /note="RNA polymerase sigma factor RpoD"
FT /id="PRO_0000093890"
FT DNA_BIND 631..650
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..506
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 515..591
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 604..658
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 460..463
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 671 AA; 77737 MW; 25992A478D90A27A CRC64;
MKKKANEEKA QKRAKTEAKA EATQENKTKE NNKAKESKIK ESKIKEAKAK EPIPVKKLSF
NEALEELFAN SLSDCVSYES IIQISAKVPT LAQIKKIKEL CQKYQKKLVS SSEYAKKLNA
IDKIKKTEEK QKVLDEELED GYDFLKEKDF LEWSRSDSPV RMYLREMGDI KLLSKDEEIE
LSKQIRLGED IILDAICSVP YLIDFIYAYK DALINRERRV KELFRSFDDD DENSVSDSKK
DEDNEEDEEN EERKKVVSEK DKKRVEKVQE SFKALDKAKK EWLKALEAPI DEREDELVRS
LTLAYKRQTL KDRLYDLEPT SKLINELVKT METTLKSGDG FEKELKRLEY KLPLFNDTLI
ANHKKILANI TNMTKEDIIA QVPEATMVSV YMDLKKLFLT KEASEEGFDL APNKLKEILE
QIKRGKLISD RAKNKMAKSN LRLVVSIAKR FTSRGLPFLD LIQEGNIGLM KAVDKFEHEK
GFKFSTYATW WIKQAISRAI ADQARTIRIP IHMIDTINRI NKVMRKHIQE NGKEPDLEVV
AEEVGLSLDK VKNVIKVTKE PISLETPVGN DDDGKFGDFV EDKNIVSSID HIMREDLKAQ
IESVLDQLNE REKAVIRMRF GLLDDESDRT LEEIGKELNV TRERVRQIES SAIKKLRSPQ
YGRILRNYLR I
