RPOD_MYXXA
ID RPOD_MYXXA Reviewed; 708 AA.
AC P17531;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-80;
GN Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=asgC, sigA;
OS Myxococcus xanthus.
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=34;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FB / DZF1;
RX PubMed=2104614; DOI=10.1128/jb.172.1.80-85.1990;
RA Inouye S.;
RT "Cloning and DNA sequence of the gene coding for the major sigma factor
RT from Myxococcus xanthus.";
RL J. Bacteriol. 172:80-85(1990).
RN [2]
RP FUNCTION, AND ASGC767 VARIANT.
RC STRAIN=DK5060;
RX PubMed=8825098; DOI=10.1111/j.1365-2958.1995.18050943.x;
RA Davis J.M., Mayor J., Plamann L.;
RT "A missense mutation in rpoD results in an A-signalling defect in
RT Myxococcus xanthus.";
RL Mol. Microbiol. 18:943-952(1995).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth (By similarity). Also required for extracellular A-
CC signal production. The A-signal is the first in a series of
CC extracellular signals that are required for myxspore differentiation in
CC M.xanthus. It is thought to act as a cell-density signal during early
CC development to ensure there are enough cells present to complete
CC fruiting-body morphogenesis. A variant at residue 598 prevents A-signal
CC production in an unknown fashion. {ECO:0000255|HAMAP-Rule:MF_00963,
CC ECO:0000269|PubMed:8825098}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; U20669; AAB60208.1; -; Genomic_DNA.
DR EMBL; M32347; AAA25404.1; -; Genomic_DNA.
DR PIR; S70834; S70834.
DR RefSeq; WP_011555174.1; NZ_JABFNT010000116.1.
DR AlphaFoldDB; P17531; -.
DR SMR; P17531; -.
DR GeneID; 41362486; -.
DR OMA; RDAKKEM; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04546; Sigma70_ner; 1.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Fruiting body; Sigma factor; Sporulation;
KW Transcription; Transcription regulation.
FT CHAIN 1..708
FT /note="RNA polymerase sigma factor RpoD"
FT /id="PRO_0000093903"
FT DNA_BIND 668..687
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 57..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 160..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..544
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 553..629
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 642..695
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT MOTIF 498..501
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 12..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..190
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 598
FT /note="E -> K (in allele asgC767; unable to produce the A-
FT signal, otherwise growth is unaffected)"
SQ SEQUENCE 708 AA; 80399 MW; 0942B5FD5642FD1B CRC64;
MPTQKPSKAS VKPTKKKVDP VIRKKKAPDG AAAKGAKTGA EEKEELVARD VVAEATEKLK
KKKKGVAPAM GDDVDPEEAA EEAAAAVQVD PDAVEDDIEE DPVAERKEVK DLLAAGREKG
FLTYDEVNDA LPADIVSSDQ IDDVMSMFGD NDIEIVDAQK AAQNNEIKPT VTVEEEKEDA
DEDEKDEDDE PGGKSNDPVR LYLRKMGSVS LLTREGEVEI AKRIEDGEKE VLRALLACKV
AVEEILDIGN KLKTAKLRVR DVIKDAPEET QSEGAEEAPE EVGEGDAPAQ LAQSELNKIE
QICKQIERFR KFAQDCDVLE EELSSKKKLT EVRKKEVKQE IKDLRTKMME VLEEMRLNKK
QVDRIVINLK ALIERVDKAE DELRDLERRY DCSMKELRPQ LKESRENPNI GKKLQKQLNL
TPEQLEVLDR DVRTAVRKIK KVEEEANLPV ESLRRNYDAI RLGERRAERA KSELVEANLR
LVVSIAKKYT NRGLQFLDLI QEGNIGLMKA VDKFEYKRGY KFSTYATWWI RQAITRAIAD
QARTIRIPVH MIETINKLIR TSRYLVQEIG REPTPEEIAE KMELPLDKVR KVLKIAKEPI
SLETPIGEEE DSHLGDFIED KSLVSPADAV INMNLAEQTR KVLATLTPRE EKVLRMRFGI
GEKSDHTLEE VGQDFEVTRE RIRQIEAKAL RKLRHPSRSK RLRSFVES
