ID   RPOD_PSEAE              Reviewed;         617 AA.
AC   P26480;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoDA;
GN   OrderedLocusNames=PA0576;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1902749; DOI=10.1016/0167-4781(91)90092-z;
RA   Tanaka K., Takahashi H.;
RT   "Cloning and analysis of the gene (rpoDA) for the principal sigma factor of
RT   Pseudomonas aeruginosa.";
RL   Biochim. Biophys. Acta 1089:113-119(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; D90118; BAA14146.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG03965.1; -; Genomic_DNA.
DR   PIR; S15900; RNPS7A.
DR   RefSeq; NP_249267.1; NC_002516.2.
DR   RefSeq; WP_003085035.1; NZ_QZGE01000010.1.
DR   AlphaFoldDB; P26480; -.
DR   SMR; P26480; -.
DR   IntAct; P26480; 3.
DR   STRING; 287.DR97_3545; -.
DR   PaxDb; P26480; -.
DR   PRIDE; P26480; -.
DR   EnsemblBacteria; AAG03965; AAG03965; PA0576.
DR   GeneID; 882038; -.
DR   KEGG; pae:PA0576; -.
DR   PATRIC; fig|208964.12.peg.610; -.
DR   PseudoCAP; PA0576; -.
DR   HOGENOM; CLU_014793_7_2_6; -.
DR   InParanoid; P26480; -.
DR   OMA; RDAKKEM; -.
DR   PhylomeDB; P26480; -.
DR   BioCyc; PAER208964:G1FZ6-583-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04546; Sigma70_ner; 1.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..617
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093905"
FT   DNA_BIND        577..596
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          170..220
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          383..453
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          462..538
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          551..604
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           407..410
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   COMPBIAS        193..216
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   617 AA;  69644 MW;  D82C2049DADCC24F CRC64;
     MSGKAQQQSR LKELIARGRE QGYLTYAEVN DHLPEDISDP EQVEDIIRMI NDMGINVFET
     APDADALLLA EADTDEAAAE EAAAALAAVE SDIGRTTDPV RMYMREMGTV ELLTREGEIE
     IAKRIEEGIR EVMSAIAQFP GTVDSILADY NRIVAEGGRL SDVLSGYIDP DDGSLPAEEV
     EPVNLKDDSA DSKEKDDEEE ESDDSSDSDD EGDGGPDPEE ARLRFTAVSE QLDKAKKALK
     KHGRGSKQAT AELTGLAELF MPIKLVPKQF DALVARVRSA LEGVRAQERA IMQLCVRDAR
     MPRADFLRLF PNHETDEKWV DSVLKSKPKY AEAIERLRDD ILRNQQKLAA LESEVELTVA
     EIKEINRAMS IGEAKARRAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV
     DKFEYRRGYK FSTYATWWIR QAITRSIADQ ARTIRIPVHM IETINKLNRI SRQMLQEMGR
     EPTPEELGER MDMPEDKIRK VLKIAKEPIS METPIGDDED SHLGDFIEDS TMQSPIEMAT
     SESLKESTRE VLAGLTAREA KVLRMRFGID MNTDHTLEEV GKQFDVTRER IRQIEAKALR
     KLRHPSRSEH LRSFLDE