ID   RPOD_PSEPH              Reviewed;         615 AA.
AC   P52326;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963};
OS   Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CFBP 6595 / CHA0).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1124983;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 19095 / LMG 27888 / CFBP 6595 / CHA0;
RX   PubMed=7665535; DOI=10.1128/jb.177.18.5387-5392.1995;
RA   Schnider U., Keel C., Blumer C., Troxler J., Defago G., Haas D.;
RT   "Amplification of the housekeeping sigma factor in Pseudomonas fluorescens
RT   CHA0 enhances antibiotic production and improves biocontrol abilities.";
RL   J. Bacteriol. 177:5387-5392(1995).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; X84416; CAA59134.1; -; Genomic_DNA.
DR   PIR; S58230; S58230.
DR   RefSeq; WP_011063840.1; NZ_LS999205.1.
DR   AlphaFoldDB; P52326; -.
DR   SMR; P52326; -.
DR   STRING; 1124983.PFLCHA0_c56160; -.
DR   PRIDE; P52326; -.
DR   GeneID; 57478613; -.
DR   PATRIC; fig|1124983.3.peg.5643; -.
DR   eggNOG; COG0568; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04546; Sigma70_ner; 1.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..615
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093906"
FT   DNA_BIND        575..594
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          166..216
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          381..451
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          460..536
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          549..602
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           405..408
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   COMPBIAS        195..213
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   615 AA;  69437 MW;  343B44664A56B095 CRC64;
     MSGKAQQQSR LKELISRGRE QGYLTYAEVN DHLPEDISDP EQVEDIIRMI NDMGINVFES
     APDADALLLA EADTDEAAAE EAAAALAAVE TDIGRTTDPV RMYMREMGTV ELLTREGEIE
     IAKRIEEGIR EVMGAIAHFP GTVDHILSEY TRVTTEGGRL SDVLSGYIDP DDGITPPAAE
     VPPPVDTKTA KADDDSEDEE AEATEDEEEA ESGPDPVIAA QRFGAVADQM EVTRKALKKH
     GRENKQAIAE MLALAELFMP IKLVPKQFEG LVERVRSALD RLRQQERAIM QLCVRDARMP
     RADFLRQFPG NEVDESWTDA LAKGKSKYAE AIARLQPDII RCQQKLTALE VETGLKIAEI
     KDINRRMSIG EAKARRAKKE MVEANLRLVI SIAKKYTNRG LQFLDLIQEG NIGLMKAVDK
     FEYRRGYKFS TYATWWIRQA ITRSIADQAR TIRIPVHMIE TINKLNRISR QMLQEMGREP
     TPEELGERME MPEDKIRKVL KIAKEPISME TPIGDDEDSH LGDFIEDSTM QSPIDVATVE
     SLKEATREVL SGLTAREAKV LRMRFGIDMN TDHTLEEVGK QFDVTRERIR QIEAKALRKL
     RHPTRSEHLR SFLDE