ID   RPOD_RHOCA              Reviewed;         674 AA.
AC   P0CZ15; P46400;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN   Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963};
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=DSM 938 / 37b4;
RX   PubMed=8918250; DOI=10.1016/0378-1119(96)00243-0;
RA   Pasternak C., Chen W., Heck C., Klug G.;
RT   "Cloning, nucleotide sequence and characterization of the rpoD gene
RT   encoding the primary sigma factor of Rhodobacter capsulatus.";
RL   Gene 176:177-184(1996).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963,
CC       ECO:0000269|PubMed:8918250}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- INDUCTION: Expression is constant during exponential and early
CC       stationary phases, and increases significantly in later stationary
CC       phase. {ECO:0000269|PubMed:8918250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR   EMBL; Z68306; CAA92648.1; -; Genomic_DNA.
DR   PIR; JC5104; JC5104.
DR   AlphaFoldDB; P0CZ15; -.
DR   SMR; P0CZ15; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04546; Sigma70_ner; 1.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..674
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093909"
FT   DNA_BIND        634..653
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          216..252
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          440..510
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          519..595
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          608..661
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           464..467
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   674 AA;  75935 MW;  36D44FAB6EB44A2A CRC64;
     MAAKDIDDTK PDTAADEDAS FDMSQAAVKR MIGEAKERGY ITIDQLNAVM PPETVSGEQI
     EDVMSMLSEM GINVVEGEEV EESEGGEVVE TGSGSREIAV AGAAGETLDR TDDPVRMYLR
     EMGSVELLSR EGEIAIAKRI EAGRNTMIAG LCESPLTFQA ITIWRDELLS EEILLRDVID
     LEATFGRSLD GDEGLEGMEG IEGPVVEGLD LEAAEGAAPA ARRPASDEPE YDADGNPISR
     IDEEEDDDDS SNMSLAAMEA ALKPKVLETL ELIARDYAKL AEMQDLRMSA TLNEDGTFTV
     AEEAAYQKLR SEIVLLVNEL HLHNNRIEAL IDQLYGINRK IMSIDSGMVK LADAARINRR
     EFIDEYRGYE LDPTWMDRMS AKPARAWVTL FEKSRHKVED LRHEMAQVGQ YVGVDISEFR
     RIVNQVQKGE KEARQAKKEM VEANLRLVIS IAKKYTNRGL QFLDLIQEGN IGLMKAVDKF
     EYRRGYKFST YATWWIRQAI TRSIADQART IRIPVHMIET INKLVRTGRQ MLHEIGREPT
     PEELAEKLQM PLEKVRKVMK IAKEPISLET PIGHEEDSQL GDFIEDKNAI LPLDSAIQEN
     LKETTTRVLP SLTPREERVL RMRFGIGMNT DHTLEEVGQQ FSVTRERIRQ IEAKALRKLK
     HPSRSRKLPS FLDQ