RPOD_RICPR
ID RPOD_RICPR Reviewed; 635 AA.
AC P33451;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=RNA polymerase sigma factor RpoD {ECO:0000255|HAMAP-Rule:MF_00963};
DE AltName: Full=Sigma-70 {ECO:0000255|HAMAP-Rule:MF_00963};
GN Name=rpoD {ECO:0000255|HAMAP-Rule:MF_00963}; OrderedLocusNames=RP858;
OS Rickettsia prowazekii (strain Madrid E).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX NCBI_TaxID=272947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Madrid E;
RX PubMed=1427089; DOI=10.1016/0378-1119(92)90175-o;
RA Marks G.L., Winkler H.H., Wood D.O.;
RT "Isolation and characterization of the gene coding for the major sigma
RT factor of Rickettsia prowazekii DNA-dependent RNA polymerase.";
RL Gene 121:155-160(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Madrid E;
RX PubMed=9823893; DOI=10.1038/24094;
RA Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA Kurland C.G.;
RT "The genome sequence of Rickettsia prowazekii and the origin of
RT mitochondria.";
RL Nature 396:133-140(1998).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00963}.
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DR EMBL; U02878; AAB81404.1; -; Unassigned_DNA.
DR EMBL; AJ235273; CAA15282.1; -; Genomic_DNA.
DR PIR; JC1379; RNRE7P.
DR RefSeq; NP_221206.1; NC_000963.1.
DR RefSeq; WP_004596762.1; NC_000963.1.
DR AlphaFoldDB; P33451; -.
DR SMR; P33451; -.
DR STRING; 272947.RP858; -.
DR EnsemblBacteria; CAA15282; CAA15282; CAA15282.
DR GeneID; 57569981; -.
DR KEGG; rpr:RP858; -.
DR PATRIC; fig|272947.5.peg.897; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_7_2_5; -.
DR OMA; HDSIWEM; -.
DR Proteomes; UP000002480; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 1.10.220.120; -; 1.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007631; RNA_pol_sigma_70_non-ess.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04546; Sigma70_ner; 1.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..635
FT /note="RNA polymerase sigma factor RpoD"
FT /id="PRO_0000093911"
FT DNA_BIND 581..600
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 182..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..456
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 465..542
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 555..608
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT REGION 610..635
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 410..413
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 635 AA; 73080 MW; 3E53A36C804505DD CRC64;
MSNSNIDNDP AKIDSLLKKA KSKKIPVTYD DINKALPLNK NPSIRQLEEA ILKFSDAGVD
ILESNEDDEI KLDIGMDEEF KLSTNVDNES EDEVEEENIG STDDPVRLYL KDMGGVDLLT
RENEVEIAKR IEEGRKTMTA SLCRSPIAMR CFIVWYEDLV NEKMLLRDLI DLEANMLHDE
VHENDEEHNS ETEVEEHEDN HLSMSRVETQ ILHNIIDRMK KISFICEELL IEAKKCYEES
FEPKVLQNSK KYNNNLELLI NEVSEIHFNS KRTEEILGKM YGINRDLINK ETAFLKLAEK
YGVTRQNFLD EYIGSVINAA WKEKMLKNKK VAWKELITKE SDYIDQMINE LSVIESNTGL
LVNDFKKLVN TIQKSERQTL QAKKDMIEAN LRLVISIAKK YANRGLQFLD LIQEGNIGLM
KAVDKFEYRR GYKFSTYATW WIRQAITRAI ADQARTIRIP VHMIETINKI LRTSRQMLNE
LGYEPTATEI ANRLSMPLDK VRKVMKIAKE PISLENPVGD DSDGGQLGDF IEDKNAVAPI
DAAIQSNLRE VTTRVLATLT PREERVLRMR FGIGMNTDHT LEEVGQQFKV TRERIRQIES
KALRKLQHPI RSKKLNSFRN GGKRGDGNPS DLLEA
