ID   RPOD_SERMA              Reviewed;         215 AA.
AC   P43344;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   23-FEB-2022, entry version 100.
DE   RecName: Full=RNA polymerase sigma factor RpoD;
DE   AltName: Full=Sigma-70;
DE   Flags: Fragment;
GN   Name=rpoD;
OS   Serratia marcescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1354954; DOI=10.1128/aac.36.7.1447;
RA   Shaw K.J., Rather P.N., Sabatelli F.J., Mann P., Munayyer H., Mierzwa R.,
RA   Petrikkos G.L., Hare R.S., Miller G.H., Bennett P.;
RT   "Characterization of the chromosomal aac(6')-Ic gene from Serratia
RT   marcescens.";
RL   Antimicrob. Agents Chemother. 36:1447-1455(1992).
RN   [2]
RP   IDENTIFICATION, AND CONCEPTUAL TRANSLATION.
RA   Rudd K.E.;
RL   Unpublished observations (JUN-1995).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=M94066; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M94066; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   STRING; 273526.SMDB11_3532; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           <1..215
FT                   /note="RNA polymerase sigma factor RpoD"
FT                   /id="PRO_0000093922"
FT   DNA_BIND        175..194
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          <1..51
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000250"
FT   REGION          60..136
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000250"
FT   REGION          149..202
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000250"
FT   MOTIF           5..8
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   NON_TER         1
SQ   SEQUENCE   215 AA;  24992 MW;  016929E4BE25E3D2 CRC64;
     LQFLDLIQEG NIGLMKAVDK FEYRRGYKFS TYATWWIRQA ITRSIADQAR TIRIPVHMIE
     TINKLNRISR QMLQEMGREP TPEELAERML MPEDKIRKVL KIAKEPISME TPIGDDEDSH
     LGDFIEDTTL ELPLDSATSE SLRSATHDVL AGLTAREXKV LRMRFGIDMN TDHTLEEVGK
     QFDVTRERIR QIEAKALRKL RHPSRSEVLR SFLDD