RPOE_BACSU
ID RPOE_BACSU Reviewed; 173 AA.
AC P12464;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=DNA-directed RNA polymerase subunit delta;
DE AltName: Full=RNAP delta factor;
GN Name=rpoE {ECO:0000303|PubMed:2843435}; OrderedLocusNames=BSU37160;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=168;
RX PubMed=2843435; DOI=10.1016/0378-1119(88)90003-0;
RA Lampe M., Binnie C., Schmidt R., Losick R.;
RT "Cloned gene encoding the delta subunit of Bacillus subtilis RNA
RT polymerase.";
RL Gene 67:13-19(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-173.
RC STRAIN=168 / JH642;
RX PubMed=2457578; DOI=10.1128/jb.170.9.4194-4208.1988;
RA Trach K., Chapman J.W., Piggot P.J., Lecoq D., Hoch J.A.;
RT "Complete sequence and transcriptional analysis of the spo0F region of the
RT Bacillus subtilis chromosome.";
RL J. Bacteriol. 170:4194-4208(1988).
RN [5]
RP SUBUNIT.
RC STRAIN=168;
RX PubMed=6802805; DOI=10.1128/jb.150.2.977-980.1982;
RA Achberger E.C., Tahara M., Whiteley H.R.;
RT "Interchangeability of delta subunits of RNA polymerase from different
RT species of the genus Bacillus.";
RL J. Bacteriol. 150:977-980(1982).
RN [6]
RP CHARACTERIZATION.
RX PubMed=7545758; DOI=10.1006/jmbi.1995.0487;
RA Lopez de Saro F.J., Woody A.Y., Helmann J.D.;
RT "Structural analysis of the Bacillus subtilis delta factor: a protein
RT polyanion which displaces RNA from RNA polymerase.";
RL J. Mol. Biol. 252:189-202(1995).
RN [7]
RP CHARACTERIZATION.
RX PubMed=10336502; DOI=10.1074/jbc.274.22.15953;
RA Lopez de Saro F.J., Yoshikawa N., Helmann J.D.;
RT "Expression, abundance, and RNA polymerase binding properties of the delta
RT factor of Bacillus subtilis.";
RL J. Biol. Chem. 274:15953-15958(1999).
RN [8]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=BS200;
RX PubMed=18289874; DOI=10.1016/j.pep.2008.01.006;
RA Yang X., Lewis P.J.;
RT "Overproduction and purification of recombinant Bacillus subtilis RNA
RT polymerase.";
RL Protein Expr. Purif. 59:86-93(2008).
RN [9]
RP SUBUNIT.
RC STRAIN=168;
RX PubMed=21710567; DOI=10.1002/pmic.201000790;
RA Delumeau O., Lecointe F., Muntel J., Guillot A., Guedon E., Monnet V.,
RA Hecker M., Becher D., Polard P., Noirot P.;
RT "The dynamic protein partnership of RNA polymerase in Bacillus subtilis.";
RL Proteomics 11:2992-3001(2011).
CC -!- FUNCTION: Participates in both the initiation and recycling phases of
CC transcription. In the presence of the delta subunit, RNAP displays an
CC increased specificity of transcription, a decreased affinity for
CC nucleic acids, and an increased efficiency of RNA synthesis because of
CC enhanced recycling. May function in sigma factor switching. It
CC displaces RNA bound to RNA polymerase in a binary complex.
CC {ECO:0000269|PubMed:10336502, ECO:0000269|PubMed:7545758}.
CC -!- SUBUNIT: RNAP is composed of a core of 2 alpha, a beta and a beta'
CC subunit. The core is associated with a delta subunit, and at least one
CC of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567).
CC When a sigma factor is associated with the core the holoenzyme is
CC formed, which can initiate transcription (PubMed:18289874).
CC {ECO:0000269|PubMed:18289874, ECO:0000269|PubMed:21710567,
CC ECO:0000269|PubMed:6802805}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype; grows as well as wild-type
CC at 25-49 degrees Celsius, no apparent impairment in sporulation or
CC phage SP01 growth. {ECO:0000269|PubMed:2843435}.
CC -!- SIMILARITY: Belongs to the RpoE family. {ECO:0000305}.
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DR EMBL; M21677; AAA22710.1; -; Genomic_DNA.
DR EMBL; Z49782; CAA89869.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15744.1; -; Genomic_DNA.
DR EMBL; M22039; AAA16800.1; -; Unassigned_DNA.
DR PIR; JT0302; JT0302.
DR RefSeq; NP_391597.1; NC_000964.3.
DR RefSeq; WP_003243661.1; NZ_JNCM01000034.1.
DR PDB; 2KRC; NMR; -; A=2-92.
DR PDB; 2M4K; NMR; -; A=2-173.
DR PDB; 4NC7; X-ray; 2.00 A; A/B=2-92.
DR PDB; 4NC8; X-ray; 2.17 A; A/B=2-92.
DR PDB; 6ZCA; EM; 4.20 A; D=1-92.
DR PDB; 6ZFB; EM; 3.90 A; D/d=1-173.
DR PDB; 7F75; EM; 4.20 A; L=1-173.
DR PDBsum; 2KRC; -.
DR PDBsum; 2M4K; -.
DR PDBsum; 4NC7; -.
DR PDBsum; 4NC8; -.
DR PDBsum; 6ZCA; -.
DR PDBsum; 6ZFB; -.
DR PDBsum; 7F75; -.
DR AlphaFoldDB; P12464; -.
DR BMRB; P12464; -.
DR SASBDB; P12464; -.
DR SMR; P12464; -.
DR STRING; 224308.BSU37160; -.
DR PaxDb; P12464; -.
DR PRIDE; P12464; -.
DR EnsemblBacteria; CAB15744; CAB15744; BSU_37160.
DR GeneID; 937046; -.
DR KEGG; bsu:BSU37160; -.
DR PATRIC; fig|224308.179.peg.4025; -.
DR eggNOG; COG3343; Bacteria.
DR OMA; TWGLRSW; -.
DR PhylomeDB; P12464; -.
DR BioCyc; BSUB:BSU37160-MON; -.
DR EvolutionaryTrace; P12464; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.10.1250; -; 1.
DR HAMAP; MF_00357; RNApol_bact_RpoE; 1.
DR InterPro; IPR007759; Asxl_HARE-HTH.
DR InterPro; IPR038087; RNAP_delta_N_dom_sf.
DR InterPro; IPR029757; RpoE.
DR Pfam; PF05066; HARE-HTH; 1.
DR TIGRFAMs; TIGR04567; RNAP_delt_lowGC; 1.
DR PROSITE; PS51913; HTH_HARE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-directed RNA polymerase;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2843435"
FT CHAIN 2..173
FT /note="DNA-directed RNA polymerase subunit delta"
FT /id="PRO_0000204312"
FT DOMAIN 14..81
FT /note="HTH HARE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01261"
FT REGION 110..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT TURN 3..5
FT /evidence="ECO:0007829|PDB:2KRC"
FT HELIX 8..13
FT /evidence="ECO:0007829|PDB:4NC7"
FT HELIX 16..27
FT /evidence="ECO:0007829|PDB:4NC7"
FT HELIX 33..43
FT /evidence="ECO:0007829|PDB:4NC7"
FT HELIX 48..54
FT /evidence="ECO:0007829|PDB:4NC7"
FT HELIX 55..64
FT /evidence="ECO:0007829|PDB:4NC7"
FT STRAND 69..77
FT /evidence="ECO:0007829|PDB:2KRC"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:4NC7"
FT STRAND 82..85
FT /evidence="ECO:0007829|PDB:2KRC"
SQ SEQUENCE 173 AA; 20399 MW; 0F5818802634417D CRC64;
MGIKQYSQEE LKEMALVEIA HELFEEHKKP VPFQELLNEI ASLLGVKKEE LGDRIAQFYT
DLNIDGRFLA LSDQTWGLRS WYPYDQLDEE TQPTVKAKKK KAKKAVEEDL DLDEFEEIDE
DDLDLDEVEE ELDLEADDFD EEDLDEDDDD LEIEEDIIDE DDEDYDDEEE EIK
