ID   RPOE_STRAT              Reviewed;         180 AA.
AC   O68887;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=RNA polymerase sigma-E factor;
GN   Name=sigE;
OS   Streptomyces antibioticus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1890;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ROLE IN THE PRODUCTION OF
RP   ACTINOMYCIN.
RC   STRAIN=IMRU3720;
RX   PubMed=9004230; DOI=10.1046/j.1365-2958.1997.2001566.x;
RA   Jones G.H., Paget M.S., Chamberlin L., Buttner M.J.;
RT   "Sigma-E is required for the production of the antibiotic actinomycin in
RT   Streptomyces antibioticus.";
RL   Mol. Microbiol. 23:169-178(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IMRU3720;
RX   PubMed=11342219; DOI=10.1016/s0167-4781(00)00257-8;
RA   Bralley P., Jones G.H.;
RT   "Transcriptional analysis and regulation of the sigma-E gene of
RT   Streptomyces antibioticus.";
RL   Biochim. Biophys. Acta 1517:410-415(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is required for the synthesis of the
CC       antibiotic actinomycin. {ECO:0000269|PubMed:9004230}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF056199; AAC14121.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68887; -.
DR   SMR; O68887; -.
DR   STRING; 1890.AFM16_21765; -.
DR   PRIDE; O68887; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0006950; P:response to stress; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014325; RNA_pol_sigma-E_actinobac.
DR   InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02983; SigE-fam_strep; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS01063; SIGMA70_ECF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..180
FT                   /note="RNA polymerase sigma-E factor"
FT                   /id="PRO_0000314476"
FT   DNA_BIND        130..149
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           36..49
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   180 AA;  20570 MW;  2CA1A4BC5FF77160 CRC64;
     MAQGEVLEFE EIVRTRQDAL LRTPPGLVPD PVDAQDLLQT ALARTYGRWE GIADKRLADA
     YLRRVMINTR TEWWRARKLE EVPTEQLPDA RVEDSTEQHA DRALLMDAMK VLAPKQRSVV
     VLRHWEQMST EETAAALGMS AGTVKSTLHR ALARLREELE TRDLDARALE REERERCAAA