ID   RPOF_STRCO              Reviewed;         287 AA.
AC   P37971;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=RNA polymerase sigma-F factor;
GN   Name=sigF; Synonyms=rpoX; OrderedLocusNames=SCO4035; ORFNames=2SCD60.01c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A3(2) / NRRL B-16638;
RX   PubMed=7476207; DOI=10.1111/j.1365-2958.1995.mmi_17010037.x;
RA   Potuckova L., Kelemen G.H., Findlay K.C., Lonetto M.A., Buttner M.J.,
RA   Kormanec J.;
RT   "A new RNA polymerase sigma factor, sigma F, is required for the late
RT   stages of morphological differentiation in Streptomyces spp.";
RL   Mol. Microbiol. 17:37-48(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is required for normal spore maturation.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. SigB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L11648; AAA93052.1; -; Genomic_DNA.
DR   EMBL; AL939118; CAC32306.1; -; Genomic_DNA.
DR   PIR; S69774; S69774.
DR   RefSeq; NP_628217.1; NC_003888.3.
DR   RefSeq; WP_011029398.1; NZ_VNID01000032.1.
DR   AlphaFoldDB; P37971; -.
DR   SMR; P37971; -.
DR   IntAct; P37971; 1.
DR   STRING; 100226.SCO4035; -.
DR   GeneID; 1099471; -.
DR   KEGG; sco:SCO4035; -.
DR   PATRIC; fig|100226.15.peg.4098; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_014793_8_0_11; -.
DR   InParanoid; P37971; -.
DR   OMA; QFPTFAM; -.
DR   PhylomeDB; P37971; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014322; RNA_pol_sigma-B/F/G.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02980; SigBFG; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..287
FT                   /note="RNA polymerase sigma-F factor"
FT                   /id="PRO_0000093980"
FT   DNA_BIND        254..273
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           91..104
FT                   /note="Polymerase core binding"
FT   COMPBIAS        1..17
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   287 AA;  31788 MW;  89F3E15B57437AE3 CRC64;
     MPASTAPQAP PAPPAQAQAQ APAQAQEAPA PQRSRGADTR ALTQVLFGEL KGLAPGTPEH
     DRVRAALIEA NLPLVRYAAA RFRSRNEPME DVVQVGTIGL INAIDRFDPE RGVQFPTFAM
     PTVVGEIKRY FRDNVRTVHV PRRLHELWVQ VNSATEDLTT AFGRSPTTAE IAERLRITEE
     EVLSCIEAGR SYHATSLEAA QEGDGLPGLL DRLGYEDPAL DGVEHRDLVR HLLVQLPERE
     QRILLLRYYS NLTQSQISAE LGVSQMHVSR LLARSFQRLR SANRIDA