ID   RPOH_BUCAP              Reviewed;         285 AA.
AC   Q8KA76;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=BUsg_026;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; AE013218; AAM67597.1; -; Genomic_DNA.
DR   RefSeq; WP_011053563.1; NC_004061.1.
DR   AlphaFoldDB; Q8KA76; -.
DR   SMR; Q8KA76; -.
DR   STRING; 198804.BUsg_026; -.
DR   EnsemblBacteria; AAM67597; AAM67597; BUsg_026.
DR   KEGG; bas:BUsg_026; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_5_6; -.
DR   OMA; YQRWLAE; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..285
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093952"
FT   DNA_BIND        254..273
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          53..122
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          229..281
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           77..80
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   285 AA;  33298 MW;  FA73D1A5D725250F CRC64;
     MTNKVQILSV TALGNLDAYI RIANLWPMLS IKEEKLLTKR LRYHDDLDAA KTLILSHLRF
     VIHISRNYSG YGLLQADLIQ EGNIGLMKAV RRFNPDIGVR LVSFAVHWIK SEIHEYVLRN
     WRIVKVATTK SQRKLFFNLR KNKKRLGWFN QEEIEIVARE LGVSSEDVRE MESRMSAQDI
     TFNPFPEEDL KDGKINGNMF YLQDKTSNFA NGLEQDNWNK HTTSKLSNAL LRLDERSRNI
     IRARWLDKKE KNTLQKIANN YGISAERVRQ LEKNAMKKLK IAIEN