ID   RPOH_BUCBP              Reviewed;         287 AA.
AC   Q89B27;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2003, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=bbp_027;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; AE016826; AAO26770.1; -; Genomic_DNA.
DR   RefSeq; WP_011091171.1; NC_004545.1.
DR   AlphaFoldDB; Q89B27; -.
DR   SMR; Q89B27; -.
DR   STRING; 224915.bbp_027; -.
DR   EnsemblBacteria; AAO26770; AAO26770; bbp_027.
DR   GeneID; 56470571; -.
DR   KEGG; bab:bbp_027; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_5_6; -.
DR   OMA; YQRWLAE; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..287
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093953"
FT   DNA_BIND        256..275
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          54..123
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          230..283
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           78..81
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   287 AA;  33557 MW;  C85A218200546F2D CRC64;
     MIKVETKTLP AISLGNLDSY IREANSWPML SSQEEKYLSE KLFYHGDLNA AKTLILSHLR
     FVIHVSRNYS GYGLLQADLI QEGNIGLMKA VRRFNPKIGV RLVSFAVHWI KSEIHEYVLR
     NWRIVKVATT KSQRKLFFNL RKTKQRLGWF NDHELELVAQ ELGVKREDVR EMESRMSAQD
     IALHHVPDNF REEQNFSYTI PYLKDSLSDF ANHVEEDNWE AHKTNKLSNA LSSLDERSRN
     IIHARWLDDS DHKMTLREIA HNYGISAERV RQLEKNAMKK LKVAIET