RPOH_CAUVC
ID RPOH_CAUVC Reviewed; 295 AA.
AC P0CAW9; P48194; Q45995;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=CC_3098;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-26.
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=8606189; DOI=10.1128/jb.178.7.2094-2101.1996;
RA Wu J., Newton A.;
RT "Isolation, identification, and transcriptional specificity of the heat
RT shock sigma factor sigma32 from Caulobacter crescentus.";
RL J. Bacteriol. 178:2094-2101(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of expression of
CC heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK25060.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U39791; AAC43981.1; -; Genomic_DNA.
DR EMBL; AE005673; AAK25060.1; ALT_INIT; Genomic_DNA.
DR PIR; H87632; H87632.
DR RefSeq; NP_421892.1; NC_002696.2.
DR AlphaFoldDB; P0CAW9; -.
DR SMR; P0CAW9; -.
DR STRING; 190650.CC_3098; -.
DR EnsemblBacteria; AAK25060; AAK25060; CC_3098.
DR KEGG; ccr:CC_3098; -.
DR PATRIC; fig|190650.5.peg.3105; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_5_5; -.
DR OMA; YQRWLAE; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00961; Sigma70_RpoH; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; DNA-binding; Reference proteome;
KW Sigma factor; Stress response; Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8606189"
FT CHAIN 2..295
FT /note="RNA polymerase sigma factor RpoH"
FT /id="PRO_0000093954"
FT DNA_BIND 254..273
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 52..121
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 230..281
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT MOTIF 76..79
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 295 AA; 33660 MW; F1520605C36DD7C3 CRC64;
MAVNSLSVMS PDGGLSRYLT EIRKFPMLSK DEEFMLAQRW KEHQDPQAAH KMVTSHLRLV
AKIAMGYRGY GLPIGEVISE GNVGLMQAVK KFEPEKGFRL ATYAMWWIRA SIQEYILRSW
SLVKMGTTAA QKKLFFNLRK AKSQIAAFQE GDLHPDQVSQ IATKLGVLDS EVISMNRRLS
GPDASLNAPL RADGESEWQD WLADEEQVSQ ETRVAEDEEK SLRMSLLEEA MVELTDRERH
ILTERRLKDD PTTLEELAAQ YGVSRERVRQ IEVRAFEKLQ KTMREAAIAK NMVDA
