ID   RPOH_CAUVN              Reviewed;         295 AA.
AC   B8H3C3; P48194; Q45995;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=CCNA_03195;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8606166; DOI=10.1128/jb.178.7.1919-1927.1996;
RA   Reisenauer A.M., Mohr C.D., Shapiro L.;
RT   "Regulation of a heat shock sigma32 homolog in Caulobacter crescentus.";
RL   J. Bacteriol. 178:1919-1927(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACL96660.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U37792; AAB01724.1; -; Genomic_DNA.
DR   EMBL; CP001340; ACL96660.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_002518568.1; NC_011916.1.
DR   AlphaFoldDB; B8H3C3; -.
DR   SMR; B8H3C3; -.
DR   PRIDE; B8H3C3; -.
DR   EnsemblBacteria; ACL96660; ACL96660; CCNA_03195.
DR   GeneID; 7330789; -.
DR   KEGG; ccs:CCNA_03195; -.
DR   PATRIC; fig|565050.3.peg.3122; -.
DR   HOGENOM; CLU_014793_3_5_5; -.
DR   OMA; YQRWLAE; -.
DR   OrthoDB; 1565734at2; -.
DR   PhylomeDB; B8H3C3; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..295
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000378312"
FT   DNA_BIND        254..273
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          52..121
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          230..281
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           76..79
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   CONFLICT        192
FT                   /note="A -> R (in Ref. 1; AAB01724)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   295 AA;  33660 MW;  F1520605C36DD7C3 CRC64;
     MAVNSLSVMS PDGGLSRYLT EIRKFPMLSK DEEFMLAQRW KEHQDPQAAH KMVTSHLRLV
     AKIAMGYRGY GLPIGEVISE GNVGLMQAVK KFEPEKGFRL ATYAMWWIRA SIQEYILRSW
     SLVKMGTTAA QKKLFFNLRK AKSQIAAFQE GDLHPDQVSQ IATKLGVLDS EVISMNRRLS
     GPDASLNAPL RADGESEWQD WLADEEQVSQ ETRVAEDEEK SLRMSLLEEA MVELTDRERH
     ILTERRLKDD PTTLEELAAQ YGVSRERVRQ IEVRAFEKLQ KTMREAAIAK NMVDA