RPOH_ECO57
ID RPOH_ECO57 Reviewed; 284 AA.
AC P0AGB5; P00580;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961};
GN OrderedLocusNames=Z4835, ECs4310;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of expression of
CC heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR EMBL; AE005174; AAG58570.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37733.1; -; Genomic_DNA.
DR PIR; F86013; F86013.
DR PIR; F91167; F91167.
DR RefSeq; NP_312337.1; NC_002695.1.
DR RefSeq; WP_000130217.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AGB5; -.
DR SMR; P0AGB5; -.
DR STRING; 155864.EDL933_4674; -.
DR EnsemblBacteria; AAG58570; AAG58570; Z4835.
DR EnsemblBacteria; BAB37733; BAB37733; ECs_4310.
DR GeneID; 66672655; -.
DR GeneID; 915834; -.
DR KEGG; ece:Z4835; -.
DR KEGG; ecs:ECs_4310; -.
DR PATRIC; fig|386585.9.peg.4503; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; YQRWLAE; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00961; Sigma70_RpoH; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..284
FT /note="RNA polymerase sigma factor RpoH"
FT /id="PRO_0000093958"
FT DNA_BIND 253..272
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 53..122
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 228..280
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT MOTIF 77..80
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 284 AA; 32469 MW; 628A17B4C835D00A CRC64;
MTDKMQSLAL APVGNLDSYI RAANAWPMLS ADEERALAEK LHYHGDLEAA KTLILSHLRF
VVHIARNYAG YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR LVSFAVHWIK AEIHEYVLRN
WRIVKVATTK AQRKLFFNLR KTKQRLGWFN QDEVEMVARE LGVTSKDVRE MESRMAAQDM
TFDLSSDDDS DSQPMAPVLY LQDKSSNFAD GIEDDNWEEQ AANRLTDAMQ GLDERSQDII
RARWLDEDNK STLQELADRY GVSAERVRQL EKNAMKKLRA AIEA
