RPOH_PSEAE
ID RPOH_PSEAE Reviewed; 284 AA.
AC P42378;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=PA0376;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=7728657; DOI=10.1139/m95-010;
RA Naczynski Z.M., Mueller C., Kropinski A.M.;
RT "Cloning the gene for the heat shock response positive regulator (sigma 32
RT homolog) from Pseudomonas aeruginosa.";
RL Can. J. Microbiol. 41:75-87(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=7698670; DOI=10.1016/0378-1119(94)00829-h;
RA Benvenisti L., Koby S., Rutman A., Giladi H., Yura T., Oppenheim A.B.;
RT "Cloning and primary sequence of the rpoH gene from Pseudomonas
RT aeruginosa.";
RL Gene 155:73-76(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Aramaki H., Fujita M.;
RT "Cloning and sequencing of the gene (rpoH) encoding the heat-shock sigma
RT factor from Pseudomonas aeruginosa.";
RL Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of expression of
CC heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR EMBL; U09560; AAA92723.1; -; Genomic_DNA.
DR EMBL; S77322; AAB33935.1; -; Genomic_DNA.
DR EMBL; D50052; BAA08769.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG03765.1; -; Genomic_DNA.
DR PIR; JC4040; JC4040.
DR RefSeq; NP_249067.1; NC_002516.2.
DR RefSeq; WP_003084510.1; NZ_QZGE01000016.1.
DR AlphaFoldDB; P42378; -.
DR SMR; P42378; -.
DR STRING; 287.DR97_3343; -.
DR PaxDb; P42378; -.
DR EnsemblBacteria; AAG03765; AAG03765; PA0376.
DR GeneID; 883126; -.
DR KEGG; pae:PA0376; -.
DR PATRIC; fig|208964.12.peg.396; -.
DR PseudoCAP; PA0376; -.
DR HOGENOM; CLU_014793_3_5_6; -.
DR InParanoid; P42378; -.
DR OMA; YQRWLAE; -.
DR PhylomeDB; P42378; -.
DR BioCyc; PAER208964:G1FZ6-380-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_00961; Sigma70_RpoH; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..284
FT /note="RNA polymerase sigma factor RpoH"
FT /id="PRO_0000093962"
FT DNA_BIND 253..272
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 54..123
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 229..280
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT MOTIF 78..81
FT /note="Interaction with polymerase core subunit RpoC"
FT CONFLICT 284
FT /note="A -> G (in Ref. 1; AAA92723)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 284 AA; 32581 MW; 282395EE38F21D5D CRC64;
MTTSLQPVHA LVPGANLEAY VHSVNSIPLL SPEQERELAE RLFYQQDLEA ARQMVLAHLR
FVVHIAKSYS GYGLAQADLI QEGNVGLMKA VKRFNPEMGV RLVSFAVHWI KAEIHEFILR
NWRIVKVATT KAQRKLFFNL RSQKKRLAWL NNEEVHRVAE SLGVEPREVR EMESRLTGQD
MAFDPAADAD DESAYQSPAH YLEDHRYDPA RQLEDADWSD SSSANLHEAL EGLDERSRDI
LQQRWLSEEK ATLHDLAEKY NVSAERIRQL EKNAMSKLKG RILA
