ID   RPOH_RHIRD              Reviewed;         300 AA.
AC   P50507;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961};
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IAM12544;
RX   PubMed=7501460;
RA   Nakahigashi K., Yanagi H., Yura T.;
RT   "Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs
RT   from Gram-negative bacteria: conserved mRNA and protein segments for heat
RT   shock regulation.";
RL   Nucleic Acids Res. 23:4383-4390(1995).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; D50828; BAA09439.1; -; Genomic_DNA.
DR   PIR; S60164; S60164.
DR   RefSeq; WP_003491840.1; NZ_VZPW01000003.1.
DR   AlphaFoldDB; P50507; -.
DR   SMR; P50507; -.
DR   STRING; 1082932.ATCR1_05841; -.
DR   GeneID; 60889437; -.
DR   eggNOG; COG0568; Bacteria.
DR   OrthoDB; 1565734at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..300
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093950"
FT   DNA_BIND        255..274
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          53..122
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          231..282
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           77..80
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   300 AA;  34418 MW;  15340A0D3735F0D9 CRC64;
     MARNSLPTIT AGEAGLNRYL DEIRKFPMLE PQEEYMLGKR YAEHGDRDAA HKLVTSHLRL
     VAKIAMGYRG YGLPIGEVVS EGNVGLMQAV KKFDPERGFR LATYAMWWIK ASIQEYILRS
     WSLVKMGTTA NQKRLFFNLR RLKGRIQAID DGDLKPEHVK EIATKLQVSE EEVISMNRRL
     HGDASLNAPI KASEGESGQW QDWLVDDHES QEAVLIEQDE LETRRRMLAK AMGVLNDRER
     RIFEARRLAE DPVTLEELSS EFDISRERVR QIEVRAFEKV QEAVQKEALE AARALRVVDA