ID   RPOH_SERMA              Reviewed;         285 AA.
AC   P50510;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961};
OS   Serratia marcescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 264 / 1404;
RX   PubMed=7501460;
RA   Nakahigashi K., Yanagi H., Yura T.;
RT   "Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs
RT   from Gram-negative bacteria: conserved mRNA and protein segments for heat
RT   shock regulation.";
RL   Nucleic Acids Res. 23:4383-4390(1995).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; D50831; BAA09442.1; -; Genomic_DNA.
DR   PIR; S60167; S60167.
DR   RefSeq; WP_004934345.1; NZ_WVHX01000029.1.
DR   AlphaFoldDB; P50510; -.
DR   SMR; P50510; -.
DR   STRING; 273526.SMDB11_4321; -.
DR   GeneID; 64310499; -.
DR   GeneID; 66716793; -.
DR   OrthoDB; 1565734at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..285
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093963"
FT   DNA_BIND        254..273
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          53..122
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          229..281
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           77..80
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   285 AA;  32671 MW;  BDBBE6C0C9B00212 CRC64;
     MTKEMQTLAL VPQGSLEAYI RAANAYPMLT AEEERELAER LHYQGDLDAA KQLILSHLRF
     VAHIARNYSG YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR LVSFAVHWIK AEIHEYVLRN
     WRIVKVATTK AQRKLFFNLR KTKQRLGWFN QDEVELVARE LGVTSKDVRE MESRMAAQDM
     TFDPTPDDEA RDGQSMAPVL YLQDKSSDFA EGIEEDNWES NAADKLAYAL EGLDERSQHI
     IRARWLDDDN KSTLQELADQ YGVSAERVRQ LEKNAMKKLK MAIEA