RPOH_VIBCH
ID RPOH_VIBCH Reviewed; 286 AA.
AC P50511; Q9KVJ3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=VC_0150;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25870 / Classical Inaba 569B / Serotype O1;
RX PubMed=9168128; DOI=10.1016/s0378-1119(96)00849-9;
RA Sahu G.K., Chowdhury R., Das J.;
RT "The rpoH gene encoding sigma 32 homolog of Vibrio cholerae.";
RL Gene 189:203-207(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of expression of
CC heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR EMBL; U44432; AAC45341.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF93327.1; -; Genomic_DNA.
DR PIR; D82357; D82357.
DR RefSeq; NP_229808.1; NC_002505.1.
DR RefSeq; WP_000184359.1; NZ_LT906614.1.
DR AlphaFoldDB; P50511; -.
DR SMR; P50511; -.
DR STRING; 243277.VC_0150; -.
DR DNASU; 2614865; -.
DR EnsemblBacteria; AAF93327; AAF93327; VC_0150.
DR GeneID; 57836438; -.
DR KEGG; vch:VC_0150; -.
DR PATRIC; fig|243277.26.peg.138; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; YQRWLAE; -.
DR BioCyc; VCHO:VC0150-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_00961; Sigma70_RpoH; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..286
FT /note="RNA polymerase sigma factor RpoH"
FT /id="PRO_0000093964"
FT DNA_BIND 254..273
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 53..122
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT REGION 229..281
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT MOTIF 77..80
FT /note="Interaction with polymerase core subunit RpoC"
FT CONFLICT 235..236
FT /note="ER -> DG (in Ref. 1; AAC45341)"
FT /evidence="ECO:0000305"
FT CONFLICT 259..260
FT /note="EM -> AK (in Ref. 1; AAC45341)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 286 AA; 32650 MW; B74C0DB72EC2F23E CRC64;
MTNQAYPMAL VSQDSLDSYI RSVNGYPMLS ADEERELAER LHYKGDIDAA KGLILSHLRF
VVHVARGYSG YGLPMADLVQ EGNIGLMKAV KRFNPEMGVR LVSFAVHWIK AEIHEYVLRN
WRIVKIATTK AQRKLFFNLR KSKKRLGWFN NGEVETVARE LGVEPAEVRE MESRLAAQDA
AFEMSAEDDE NGMAYTAPVL YLEDKHSDLA DNLEAENWEA HTTQRLSMAL ASLDERSQHI
VRARWLDDDN KTTLQDLAEM YGVSAERIRQ LEKNAMRKLK EAVGEF
