ID   RPOH_ZYMMO              Reviewed;         302 AA.
AC   P50512; Q5NPI7;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 2.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=ZMO0749;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=264203;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RX   PubMed=7501460;
RA   Nakahigashi K., Yanagi H., Yura T.;
RT   "Isolation and sequence analysis of rpoH genes encoding sigma 32 homologs
RT   from Gram-negative bacteria: conserved mRNA and protein segments for heat
RT   shock regulation.";
RL   Nucleic Acids Res. 23:4383-4390(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RX   PubMed=15592456; DOI=10.1038/nbt1045;
RA   Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA   Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA   Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA   Kang H.S.;
RT   "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT   ZM4.";
RL   Nat. Biotechnol. 23:63-68(2005).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; D50832; BAA09444.1; -; Genomic_DNA.
DR   EMBL; AE008692; AAV89373.1; -; Genomic_DNA.
DR   PIR; S60169; S60169.
DR   RefSeq; WP_011240630.1; NZ_CP035711.1.
DR   AlphaFoldDB; P50512; -.
DR   SMR; P50512; -.
DR   STRING; 264203.ZMO0749; -.
DR   EnsemblBacteria; AAV89373; AAV89373; ZMO0749.
DR   GeneID; 58026568; -.
DR   KEGG; zmo:ZMO0749; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_5_5; -.
DR   OMA; YQRWLAE; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000001173; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02392; rpoH_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..302
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093966"
FT   DNA_BIND        259..278
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          57..126
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          235..286
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           81..84
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   CONFLICT        238
FT                   /note="K -> R (in Ref. 1; BAA09444)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   302 AA;  34435 MW;  15811A87E38D4D52 CRC64;
     MATSSTLPAV VPALGGDQSL NHYLADIRKF PILKPEEEYM LAKRFQEHQD PKAASRLVTS
     HLRLVAKIAM GYRGYGLPVS ELISEGNIGL MQGVKKFDPE RGFRLATYAI WWIKASIQEY
     ILRSWSLVKM GTTAAQKKLF FNLRRLKSNM NAFEDGDLKP DEVDSIATNL GVSNEEVVNM
     NRRMAMGGDS SLNITMREDG EGQMQDWLVD QEPLQDQQIE EEEESLVRHK LLIEAMDKLN
     DREKHILTER RLSDNPKTLE ELSQVYGVSR ERVRQIEVRA FDKLQKAIME LAGDRKLLPA
     MA