RPOLV_BPN4
ID RPOLV_BPN4 Reviewed; 3500 AA.
AC Q859P9;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Virion DNA-directed RNA polymerase {ECO:0000312|EMBL:AAO24831.2};
DE Short=vRNAP;
DE EC=2.7.7.6 {ECO:0000269|PubMed:21321236};
DE AltName: Full=Gene product 50 {ECO:0000305};
DE Short=gp50;
GN Name=50;
OS Enterobacteria phage N4 (Bacteriophage N4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Schitoviridae; Enquatrovirinae; Enquatrovirus.
OX NCBI_TaxID=10752 {ECO:0000312|Proteomes:UP000001789};
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Hendrix R.W., Rothman-Denes L., Hatfull G.F., Lawrence J.G., Pedulla M.;
RT "Genome sequence and analysis of bacteriophage N4.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=322130; DOI=10.1073/pnas.74.2.520;
RA Falco S.C., Laan K.V., Rothman-Denes L.B.;
RT "Virion-associated RNA polymerase required for bacteriophage N4
RT development.";
RL Proc. Natl. Acad. Sci. U.S.A. 74:520-523(1977).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=18374942; DOI=10.1016/j.jmb.2008.02.059;
RA Choi K.H., McPartland J., Kaganman I., Bowman V.D., Rothman-Denes L.B.,
RA Rossmann M.G.;
RT "Insight into DNA and protein transport in double-stranded DNA viruses: the
RT structure of bacteriophage N4.";
RL J. Mol. Biol. 378:726-736(2008).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2102, AND FUNCTION.
RX PubMed=19061645; DOI=10.1016/j.molcel.2008.11.010;
RA Gleghorn M.L., Davydova E.K., Rothman-Denes L.B., Murakami K.S.;
RT "Structural basis for DNA-hairpin promoter recognition by the bacteriophage
RT N4 virion RNA polymerase.";
RL Mol. Cell 32:707-717(2008).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2101, AND FUNCTION.
RX PubMed=18362338; DOI=10.1073/pnas.0712325105;
RA Murakami K.S., Davydova E.K., Rothman-Denes L.B.;
RT "X-ray crystal structure of the polymerase domain of the bacteriophage N4
RT virion RNA polymerase.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:5046-5051(2008).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 998-2103 IN COMPLEX WITH ATP AND
RP MANGANESE, AND COFACTOR.
RX PubMed=21321236; DOI=10.1073/pnas.1016691108;
RA Gleghorn M.L., Davydova E.K., Basu R., Rothman-Denes L.B., Murakami K.S.;
RT "X-ray crystal structures elucidate the nucleotidyl transfer reaction of
RT transcript initiation using two nucleotides.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:3566-3571(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2103 IN COMPLEX WITH ATP AND
RP MANGANESE, AND COFACTOR.
RX PubMed=23235152; DOI=10.1074/jbc.m112.387712;
RA Basu R.S., Murakami K.S.;
RT "Watching the bacteriophage N4 RNA polymerase transcription by time-
RT dependent soak-trigger-freeze X-ray crystallography.";
RL J. Biol. Chem. 288:3305-3311(2013).
CC -!- FUNCTION: DNA-dependent RNA polymerase, which is injected into the host
CC upon infection and transcribes the phage early genes from promoters
CC that have a 5-bp stem-3 nt loop hairpin structure.
CC {ECO:0000269|PubMed:18362338, ECO:0000269|PubMed:19061645}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000269|PubMed:21321236};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:21321236};
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:18374942,
CC ECO:0000269|PubMed:322130}. Note=Present in about 4 copies in the
CC virion. {ECO:0000269|PubMed:18374942}.
CC -!- DOMAIN: Contains three functional domains; the central domain possesses
CC the transcriptional activity. {ECO:0000269|PubMed:18362338}.
CC -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC family. {ECO:0000305}.
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DR EMBL; EF056009; AAO24831.2; -; Genomic_DNA.
DR RefSeq; YP_950528.1; NC_008720.1.
DR PDB; 2PO4; X-ray; 2.00 A; A=998-2101.
DR PDB; 3C2P; X-ray; 2.00 A; A/B=998-2102.
DR PDB; 3C3L; X-ray; 2.40 A; A/B=998-2102.
DR PDB; 3C46; X-ray; 2.00 A; A/B=998-2102.
DR PDB; 3Q0A; X-ray; 2.69 A; A/B=998-2103.
DR PDB; 3Q22; X-ray; 2.11 A; A/B=998-2103.
DR PDB; 3Q23; X-ray; 1.80 A; A/B=998-2103.
DR PDB; 3Q24; X-ray; 1.81 A; A/B=998-2102.
DR PDB; 4FF1; X-ray; 2.47 A; A/B=998-2103.
DR PDB; 4FF2; X-ray; 2.00 A; A/B=998-2103.
DR PDB; 4FF3; X-ray; 2.00 A; A/B=998-2103.
DR PDB; 4FF4; X-ray; 2.03 A; A/B=998-2103.
DR PDBsum; 2PO4; -.
DR PDBsum; 3C2P; -.
DR PDBsum; 3C3L; -.
DR PDBsum; 3C46; -.
DR PDBsum; 3Q0A; -.
DR PDBsum; 3Q22; -.
DR PDBsum; 3Q23; -.
DR PDBsum; 3Q24; -.
DR PDBsum; 4FF1; -.
DR PDBsum; 4FF2; -.
DR PDBsum; 4FF3; -.
DR PDBsum; 4FF4; -.
DR SMR; Q859P9; -.
DR PRIDE; Q859P9; -.
DR GeneID; 5075715; -.
DR KEGG; vg:5075715; -.
DR EvolutionaryTrace; Q859P9; -.
DR Proteomes; UP000001789; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Coiled coil; DNA-directed RNA polymerase;
KW GTP-binding; Magnesium; Manganese; Metal-binding; Nucleotide-binding;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase;
KW Virion.
FT CHAIN 1..3500
FT /note="Virion DNA-directed RNA polymerase"
FT /id="PRO_0000431828"
FT REGION 907..1003
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2078..2111
FT /evidence="ECO:0000255"
FT COILED 2777..2810
FT /evidence="ECO:0000255"
FT COMPBIAS 907..983
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 984..1003
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1434..1437
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000269|PubMed:21321236,
FT ECO:0000269|PubMed:23235152"
FT BINDING 1556..1561
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000269|PubMed:21321236,
FT ECO:0000269|PubMed:23235152"
FT BINDING 1556
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000269|PubMed:21321236,
FT ECO:0000269|PubMed:23235152"
FT BINDING 1667..1668
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000269|PubMed:21321236,
FT ECO:0000269|PubMed:23235152"
FT BINDING 1948
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000269|PubMed:21321236,
FT ECO:0000269|PubMed:23235152"
FT HELIX 1005..1012
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1016..1020
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1024..1026
FT /evidence="ECO:0007829|PDB:2PO4"
FT HELIX 1030..1034
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1044..1047
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1051..1059
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1062..1068
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1080..1109
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1110..1112
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1113..1118
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1123..1125
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1127..1129
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1130..1134
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1135..1138
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1141..1144
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1146..1162
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1164..1166
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1172..1179
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1183..1185
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1190..1196
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1197..1199
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1200..1215
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1225..1228
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1230..1245
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1248..1255
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1256..1258
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1266..1272
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1280..1283
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1287..1292
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1293..1295
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1311..1313
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1316..1320
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1323..1334
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1337..1339
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1341..1359
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1366..1368
FT /evidence="ECO:0007829|PDB:2PO4"
FT HELIX 1371..1401
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1405..1407
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1414..1416
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1422..1429
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1430..1432
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1434..1437
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1440..1442
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1445..1447
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1455..1467
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1472..1474
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1477..1489
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1490..1492
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1493..1505
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1512..1520
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1521..1523
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1526..1540
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1544..1546
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1547..1549
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1552..1557
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1560..1569
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1576..1583
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1584..1586
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1587..1590
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1596..1602
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1608..1625
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1626..1629
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1631..1647
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1651..1653
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1655..1657
FT /evidence="ECO:0007829|PDB:3C3L"
FT STRAND 1659..1661
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1664..1674
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1679..1707
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1713..1718
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1719..1721
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1723..1725
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1726..1744
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1745..1750
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1753..1758
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1769..1771
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1776..1789
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1791..1802
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1804..1839
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1853..1861
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1862..1866
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1869..1871
FT /evidence="ECO:0007829|PDB:3Q24"
FT STRAND 1881..1885
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1886..1888
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1890..1893
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1906..1909
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 1913..1915
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1916..1924
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1926..1935
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1944..1946
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 1949..1953
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1954..1956
FT /evidence="ECO:0007829|PDB:4FF3"
FT HELIX 1957..1971
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1976..1990
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1993..1995
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 1998..2008
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 2011..2013
FT /evidence="ECO:0007829|PDB:3Q23"
FT TURN 2014..2016
FT /evidence="ECO:0007829|PDB:3C2P"
FT HELIX 2019..2048
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 2052..2054
FT /evidence="ECO:0007829|PDB:3Q23"
FT STRAND 2056..2060
FT /evidence="ECO:0007829|PDB:3Q23"
FT HELIX 2077..2096
FT /evidence="ECO:0007829|PDB:3Q23"
SQ SEQUENCE 3500 AA; 382495 MW; B4A52EAC2E28BBDB CRC64;
MSVFDRLAGF ADSVTNAKQV DVSTATAQKK AEQGVTTPLV SPDAAYQMQA ARTGNVGANA
FEPGTVQSDF MNLTPMQIMN KYGVEQGLQL INARADAGNQ VFNDSVTTRT PGEELGDIAT
GVGLGFVNTL GGIGALGAGL LNDDAGAVVA QQLSKFNDAV HATQSQALQD KRKLFAARNL
MNEVESERQY QTDKKEGTND IVASLSKFGR DFVGSIENAA QTDSIISDGL AEGVGSLLGA
GPVLRGASLL GKAVVPANTL RSAALAGAID AGTGTQSLAR IASTVGRAAP GMVGVGAMEA
GGAYQQTADE IMKMSLKDLE KSPVYQQHIK DGMSPEQARR QTASETGLTA AAIQLPIAAA
TGPLVSRFEM APFRAGSLGA VGMNLARETV EEGVQGATGQ LAQNIAQQQN IDKNQDLLKG
VGTQAGLGAL YGFGSAGVVQ APAGAARLAG AATAPVLRTT MAGVKAAGSV AGKVVSPIKN
TLVARGERVM KQNEEASPVA DDYVAQAAQE AMAQAPEAEV TIRDAVEATD ATPEQKVAAH
QYVSDLMNAT RFNPENYQEA PEHIRNAVAG STDQVQVIQK LADLVNTLDE SNPQALMEAA
SYMYDAVSEF EQFINRDPAA LDSIPKDSPA IELLNRYTNL TANIQNTPKV IGALNVINRM
INESAQNGSL NVTEESSPQE MQNVALAAEV APEKLNPESV NVVLKHAADG RIKLNNRQIA
ALQNAAAILK GAREYDAEAA RLGLRPQDIV SKQIKTDESR TQEGQYSALQ HANRIRSAYN
SGNFELASAY LNDFMQFAQH MQNKVGALNE HLVTGNADKN KSVHYQALTA DREWVRSRTG
LGVNPYDTKS VKFAQQVALE AKTVADIANA LASAYPELKV SHIKVTPLDS RLNAPAAEVV
KAFRQGNRDV ASSQPKADSV NQVKETPVTK QEPVTSTVQT KTPVSESVKT EPTTKESSPQ
AIKEPVNQSE KQDVNLTNED NIKQPTESVK ETETSTKEST VTEELKEGID AVYPSLVGTA
DSKAEGIKNY FKLSFTLPEE QKSRTVGSEA PLKDVAQALS SRARYELFTE KETANPAFNG
EVIKRYKELM EHGEGIADIL RSRLAKFLNT KDVGKRFAQG TEANRWVGGK LLNIVEQDGD
TFKYNEQLLQ TAVLAGLQWR LTATSNTAIK DAKDVAAITG IDQALLPEGL VEQFDTGMTL
TEAVSSLAQK IESYWGLSRN PNAPLGYTKG IPTAMAAEIL AAFVESTDVV ENIVDMSEID
PDNKKTIGLY TITELDSFDP INSFPTAIEE AVLVNPTEKM FFGDDIPPVA NTQLRNPAVR
NTPEQKAALK AEQATEFYVH TPMVQFYETL GKDRILELMG AGTLNKELLN DNHAKSLEGK
NRSVEDSYNQ LFSVIEQVRA QSEDISTVPI HYAYNMTRVG RMQMLGKYNP QSAKLVREAI
LPTKATLDLS NQNNEDFSAF QLGLAQALDI KVHTMTREVM SDELTKLLEG NLKPAIDMMV
EFNTTGSLPE NAVDVLNTAL GDRKSFVALM ALMEYSRYLV AEDKSAFVTP LYVEADGVTN
GPINAMMLMT GGLFTPDWIR NIAKGGLFIG SPNKTMNEHR STADNNDLYQ ASTNALMESL
GKLRSNYASN MPIQSQIDSL LSLMDLFLPD INLGENGALE LKRGIAKNPL TITIYGSGAR
GIAGKLVSSV TDAIYERMSD VLKARAKDPN ISAAMAMFGK QAASEAHAEE LLARFLKDME
TLTSTVPVKR KGVLELQSTG TGAKGKINPK TYTIKGEQLK ALQENMLHFF VEPLRNGITQ
TVGESLVYST EQLQKATQIQ SVVLEDMFKQ RVQEKLAEKA KDPTWKKGDF LTQKELNDIQ
ASLNNLAPMI ETGSQTFYIA GSENAEVANQ VLATNLDDRM RVPMSIYAPA QAGVAGIPFM
TIGTGDGMMM QTLSTMKGAP KNTLKIFDGM NIGLNDITDA SRKANEAVYT SWQGNPIKNV
YESYAKFMKN VDFSKLSPEA LEAIGKSALE YDQRENATVD DIANAASLIE RNLRNIALGV
DIRHKVLDKV NLSIDQMAAV GAPYQNNGKI DLSNMTPEQQ ADELNKLFRE ELEARKQKVA
KARAEVKEET VSEKEPVNPD FGMVGREHKA SGVRILSATA IRNLAKISNL PSTQAATLAE
IQKSLAAKDY KIIYGTPTQV AEYARQKNVT ELTSQEMEEA QAGNIYGWTN FDDKTIYLVS
PSMETLIHEL VHASTFEEVY SFYQGNEVSP TSKQAIENLE GLMEQFRSLD ISKDSPEMRE
AYADAIATIE GHLSNGFVDP AISKAAALNE FMAWGLANRA LAAKQKRTSS LVQMVKDVYQ
AIKKLIWGRK QAPALGEDMF SNLLFNSAIL MRSQPTTQAV AKDGTLFHSK AYGNNERLSQ
LNQTFDKLVT DYLRTDPVTE VERRGNVANA LMSATRLVRD VQSHGFNMTA QEQSVFQMVT
AALATEAAID PHAMARAQEL YTHVMKHLTV EHFMADPDST NPADRYYAQQ KYDTISGANL
VEVDAKGRTS LLPTFLGLAM VNEELRSIIK EMPVPKADKK LGNDIDTLLT NAGTQVMESL
NRRMAGDQKA TNVQDSIDAL SETIMAAALK RESFYDAVAT PTGNFIDRAN QYVTDSIERL
SETVIEKADK VIANPSNIAA KGVAHLAKLT AAIASEKQGE IVAQGVMTAM NQGKVWQPFH
DLVNDIVGRT KTNANVYDLI KLVKSQISQD RQQFREHLPT VIAGKFSRKL TDTEWSAMHT
GLGKTDLAVL RETMSMAEIR DLLSSSKKVK DEISTLEKEI QNQAGRNWNL VQKKSKQLAQ
YMIMGEVGNN LLRNAHAISR LLGERITNGP VADVAAIDKL ITLYSLELMN KSDRDLLSEL
AQSEVEGMEF SIAYMVGQRT EEMRKAKGDN RTLLNHFKGY IPVENQQGVN LIIADDKEFA
KLNSQSFTRI GTYQGSTGFR TGSKGYYFSP VAARAPYSQG ILQNVRNTAG GVDIGTGFTL
GTMVAGRITD KPTVERITKA LAKGERGREP LMPIYNSKGQ VVAYEQSVDP NMLKHLNQDN
HFAKMVGVWR GRQVEEAKAQ RFNDILIEQL HAMYEKDIKD SSANKSQYVN LLGKIDDPVL
ADAINLMNIE TRHKAEELFG KDELWVRRDM LNDALGYRAA SIGDVWTGNS RWSPSTLDTV
KKMFLGAFGN KAYHVVMNAE NTIQNLVKDA KTVIVVKSVV VPAVNFLANI YQMIGRGVPV
KDIAVNIPRK TSEINQYIKS RLRQIDAEAE LRAAEGNPNL VRKLKTEIQS ITDSHRRMSI
WPLIEAGEFS SIADAGISRD DLLVAEGKIH EYMEKLANKL PEKVRNAGRY ALIAKDTALF
QGIQKTVEYS DFIAKAIIYD DLVKRKKKSS SEALGQVTEE FINYDRLPGR FRGYMESMGL
MWFYNFKIRS IKVAMSMIRN NPVHSLIATV VPAPTMFGNV GLPIQDNMLT MLAEGRLDYS
LGFGQGLRAP TLNPWFNLTH
