ID   RPOL_BPKMV              Reviewed;         816 AA.
AC   Q7Y2D9;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-directed RNA polymerase {ECO:0000303|PubMed:12890620};
DE            EC=2.7.7.6 {ECO:0000250|UniProtKB:P06221};
GN   Name=gp26 {ECO:0000312|EMBL:CAD44217.1};
OS   Pseudomonas phage phiKMV.
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus.
OX   NCBI_TaxID=204270 {ECO:0000312|Proteomes:UP000000842};
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=12890620; DOI=10.1016/s0042-6822(03)00123-5;
RA   Lavigne R., Burkal'tseva M.V., Robben J., Sykilinda N.N., Kurochkina L.P.,
RA   Grymonprez B., Jonckx B., Krylov V.N., Mesyanzhinov V.V., Volckaert G.;
RT   "The genome of bacteriophage phiKMV, a T7-like virus infecting Pseudomonas
RT   aeruginosa.";
RL   Virology 312:49-59(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase that catalyzes the transcription
CC       of viral genes. {ECO:0000250|UniProtKB:P06221,
CC       ECO:0000269|PubMed:12890620}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P06221};
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ505558; CAD44217.1; -; Genomic_DNA.
DR   RefSeq; NP_877465.1; NC_005045.1.
DR   SMR; Q7Y2D9; -.
DR   GeneID; 1482610; -.
DR   KEGG; vg:1482610; -.
DR   Proteomes; UP000000842; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0039695; P:DNA-templated viral transcription; ISS:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase; Viral transcription.
FT   CHAIN           1..816
FT                   /note="DNA-directed RNA polymerase"
FT                   /id="PRO_0000431827"
FT   ACT_SITE        517
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
FT   ACT_SITE        593
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
FT   ACT_SITE        754
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
SQ   SEQUENCE   816 AA;  91752 MW;  669BAD60B00A431C CRC64;
     MDLIQQQIAH EEALVGAAQN DARIALEKAI AQGSIDRIPR ARIMLMRMLP IVTEAIFAHQ
     EAKAAGPAAK LRHLLRIIDA QDLAVMALRA GLSMLINYPT ITATKYYTHM GKMLCREIEV
     RLAFKVNQPY YDRTLDYLKT SRTRSVRHIQ KTMDALLDAV LPEEARIDLP DGDYLRLGKF
     IGDPLIQCGL FEPNRFTGRG GTSVHLEPSP EAREFLQDPS AAMTWGGPGR SVMLAPPRPW
     NDWCDGGYYS AKAQKHHVLV RRTKHQTKRA RQAQLRHLGQ DKMPKVYEAV NVLQSVAYEI
     NRDVYEIIER VFNSGGGVLG IPQRTYPDKP EFPLGDEWAK ENASEQELEA FNRWKRSVHR
     WYTGEREHTA KLREFAALYR VVREHHGKAV YFPMHVDSRG RMYYWGTPNP QGSDIAKACL
     RFHDKRVLGK RGLYWLKVHV ANSLGCDKVY FDDRAAWVDE RWDDFQRALA EGPENYPGLF
     PEAESPLCAI AGLLELRAAY ASGNPEGYRS GFIVHMDATC SGLQHYSAIL RDEIGGAYVN
     LLPPGLAKAD IYSRVLGLVN ESLERDRAPG AEGEARGYAL LWDKAGLSRS LTKKPCMTLV
     YGTTFKGVVD HCLDYLDESG VEIPEGVPSY RLGSYMATLI LDAIRETVPS AVFAMEWLQR
     LAKALPDASK DLHWITPLGM QVFQSYPKTE EVRVRLRAEA VEYVTLYEAK DELDPMRNAN
     GIAPNFVHGL DSSHLGLTAL ACAAEDIPIQ AIHDSMGTYA SDVDRMHVHI REQFIAMYSG
     PCVLVELAKQ LGVEATPPRR GSLNLEAVRD SWAFFC