ID   RPOL_BPSP6              Reviewed;         874 AA.
AC   P06221;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=DNA-directed RNA polymerase;
DE            EC=2.7.7.6 {ECO:0000269|PubMed:3031606};
OS   Enterobacteria phage SP6 (Bacteriophage SP6).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Molineuxvirinae; Zindervirus.
OX   NCBI_TaxID=194966;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=3031606; DOI=10.1093/nar/15.6.2653;
RA   Kotani H., Ishizaki Y., Hiraoka N., Obayashi A.;
RT   "Nucleotide sequence and expression of the cloned gene of bacteriophage SP6
RT   RNA polymerase.";
RL   Nucleic Acids Res. 15:2653-2664(1987).
CC   -!- FUNCTION: DNA-dependent RNA polymerase that catalyzes the transcription
CC       of viral genes. {ECO:0000269|PubMed:3031606}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032, ECO:0000269|PubMed:3031606};
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; Y00105; CAA68288.1; -; mRNA.
DR   PIR; S07380; S07380.
DR   SMR; P06221; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase; Viral transcription.
FT   CHAIN           1..874
FT                   /note="DNA-directed RNA polymerase"
FT                   /id="PRO_0000087750"
FT   ACT_SITE        536
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
FT   ACT_SITE        623
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
FT   ACT_SITE        806
FT                   /evidence="ECO:0000250|UniProtKB:P00573"
SQ   SEQUENCE   874 AA;  98533 MW;  207BD3F3CF9B501C CRC64;
     MQDLHAIQLQ LEEEMFNGGI RRFEADQQRQ IAAGSESDTA WNRRLLSELI APMAEGIQAY
     KEEYEGKKGR APRALAFLQC VENEVAAYIT MKVVMDMLNT DATLQAIAMS VAERIEDQVR
     FSKLEGHAAK YFEKVKKSLK ASRTKSYRHA HNVAVVAEKS VAEKDADFDR WEAWPKETQL
     QIGTTLLEIL EGSVFYNGEP VFMRAMRTYG GKTIYYLQTS ESVGQWISAF KEHVAQLSPA
     YAPCVIPPRP WRTPFNGGFH TEKVASRIRL VKGNREHVRK LTQKQMPKVY KAINALQNTQ
     WQINKDVLAV IEEVIRLDLG YGVPSFKPLI DKENKPANPV PVEFQHLRGR ELKEMLSPEQ
     WQQFINWKGE CARLYTAETK RGSKSAAVVR MVGQARKYSA FESIYFVYAM DSRSRVYVQS
     STLSPQSNDL GKALLRFTEG RPVNGVEALK WFCINGANLW GWDKKTFDVR VSNVLDEEFQ
     DMCRDIAADP LTFTQWAKAD APYEFLAWCF EYAQYLDLVD EGRADEFRTH LPVHQDGSCS
     GIQHYSAMLR DEVGAKAVNL KPSDAPQDIY GAVAQVVIKK NALYMDADDA TTFTSGSVTL
     SGTELRAMAS AWDSIGITRS LTKKPVMTLP YGSTRLTCRE SVIDYIVDLE EKEAQKAVAE
     GRTANKVHPF EDDRQDYLTP GAAYNYMTAL IWPSISEVVK APIVAMKMIR QLARFAAKRN
     EGLMYTLPTG FILEQKIMAT EMLRVRTCLM GDIKMSLQVE TDIVDEAAMM GAAAPNFVHG
     HDASHLILTV CELVDKGVTS IAVIHDSFGT HADNTLTLRV ALKGQMVAMY IDGNALQKLL
     EEHEVRWMVD TGIEVPEQGE FDLNEIMDSE YVFA