ID   RPOL_KLULA              Reviewed;         982 AA.
AC   P05472;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
DE   AltName: Full=Killer plasmid PGKL2 protein 6;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Plasmid pGKl-2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3041369; DOI=10.1093/nar/16.13.5863;
RA   Tommasino M., Ricci S., Galeotti C.L.;
RT   "Genome organization of the killer plasmid pGKL2 from Kluyveromyces
RT   lactis.";
RL   Nucleic Acids Res. 16:5863-5878(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3138657;
RA   Wilson D.W., Meacock P.A.;
RT   "Extranuclear gene expression in yeast: evidence for a plasmid-encoded RNA
RT   polymerase of unique structure.";
RL   Nucleic Acids Res. 16:8097-8112(1988).
CC   -!- FUNCTION: The presence of the two linear plasmids, termed pGKL1 and
CC       pGKL2, in strains of Kluyveromyces lactis confers the killer phenotype
CC       to the host cell, by promoting the secretion of a toxin able to inhibit
CC       the growth of sensitive strains.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; X07776; CAA30607.1; -; Genomic_DNA.
DR   EMBL; X07946; CAA30770.1; -; Genomic_DNA.
DR   PIR; S00964; S00964.
DR   PIR; S10340; S10340.
DR   AlphaFoldDB; P05472; -.
DR   STRING; 284590.P05472; -.
DR   Proteomes; UP000000598; Plasmid pGKL2.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 2.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 3.
DR   SMART; SM00663; RPOLA_N; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..982
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000048071"
FT   CONFLICT        32
FT                   /note="T -> N (in Ref. 2; CAA30770)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        303
FT                   /note="I -> K (in Ref. 2; CAA30770)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        917
FT                   /note="F -> C (in Ref. 2; CAA30770)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   982 AA;  113961 MW;  E9AEF1F4581B52CF CRC64;
     MIVLDIPKMD YGQIEIYNDY FRNVDKKTIK YTPMECIEYK LSYCAIYNDR ELPIMIGSSL
     DISKENPLNL KGYFIIDGIC KSVNNIKIKE KIHFSKDRAY LSDDSIIQIK DMFNMTIKNN
     KNTLKWNLPL NWSDIIKYSD HKKELENHLK IIDSFNKKAE KPIKNEIDLI SLCYMFECWL
     GLREEPRLYY RLVTPGELIH DIIKEKRSVI KCFRTNTWTV KNIRNVFCVS EDMKHYNKIG
     DLESIRRITI PSSRSNAPMK ERRVEDNDKY KICPIQTSDG SLCGTITYLC KDAKITTKID
     TIIVEKADNG LHTFFNNTYL GKVKVKTKES DIYCIDKICY IFGNIGRIIK GNYLCSYTGE
     LLDYKNYNPP IRSMFVCNMI KQAITGDSVY SGDILHNTKS LINGDLGHKL EIAIMPWKKF
     NIEDAIVISN KVSKLFRSKR TIIHRENDVK ILNVYVNINQ NITKGDLLYR TYDPMQVKTI
     NFVYAEHDGK VKEIIREEKY LKLILIKERD LEVGDKMSSM HGQKGIVSLI ENNMPYYYKN
     GEKVEIDLII NPHAFPSRMT LGQIKEMGEK EEYVYIEDTK LENKIIVGKC LYLALRHQVD
     DKVQFRNGGN IDIVTKQPVS GRKRSGGLRF GQMERDILIG LGAWNTIKEI WSIDRSKIKI
     DSKTGRINWI RYDKEMEIGQ YFKICLSYMR SLGRDILIKN DKYSIVEFDN SYLPKTDTMK
     FGDLDVLDLR IYKGIVMLPL CLRSTYLNKL YVDRKYSEAE KEVTKLLKSK NGAYHTLVEG
     HRVDRCIRSV IVPDPTLDID TIKIPFGANI GCEYGLLNRQ PSLNVDSIKL VKLKQGSNKT
     IAINPLLCQS FNADFDGDEM NIYGIRNKES IEEMKILAKV IENKTQDYIL DKKDLTANKK
     GILEMIEKGS KGKMFNFEHM FKEIGKVTIN KKEINIKGCY NNSINDEEWY EMCKVARENA
     ASISINTPIT GYLENICNEM YL