ID   RPOM_NEUCR              Reviewed;        1423 AA.
AC   P38671; Q7RVH0;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=DNA-directed RNA polymerase, mitochondrial;
DE            EC=2.7.7.6;
DE   Flags: Precursor;
GN   Name=cyt-5; ORFNames=NCU06308;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=8626458; DOI=10.1074/jbc.271.11.6537;
RA   Chen B., Kubelik A.R., Mohr S., Breitenberger C.A.;
RT   "Cloning and characterization of the Neurospora crassa cyt-5 gene. A
RT   nuclear-coded mitochondrial RNA polymerase with a polyglutamine repeat.";
RL   J. Biol. Chem. 271:6537-6544(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; L25087; AAA33587.1; -; Genomic_DNA.
DR   EMBL; CM002239; EAA33334.3; -; Genomic_DNA.
DR   PIR; T18363; T18363.
DR   RefSeq; XP_962570.3; XM_957477.3.
DR   AlphaFoldDB; P38671; -.
DR   SMR; P38671; -.
DR   STRING; 5141.EFNCRP00000006114; -.
DR   EnsemblFungi; EAA33334; EAA33334; NCU06308.
DR   GeneID; 3878704; -.
DR   KEGG; ncr:NCU06308; -.
DR   VEuPathDB; FungiDB:NCU06308; -.
DR   HOGENOM; CLU_003364_1_0_1; -.
DR   InParanoid; P38671; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0001018; F:mitochondrial promoter sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase; Transit peptide.
FT   TRANSIT         1..73
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           74..1423
FT                   /note="DNA-directed RNA polymerase, mitochondrial"
FT                   /id="PRO_0000031076"
FT   REGION          266..303
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1055..1087
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1316..1342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        272..303
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1066..1082
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        901
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        970
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        1180
FT                   /evidence="ECO:0000250"
FT   CONFLICT        311
FT                   /note="L -> F (in Ref. 1; AAA33587)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        468..470
FT                   /note="GGA -> RS (in Ref. 1; AAA33587)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        752..753
FT                   /note="LH -> FD (in Ref. 1; AAA33587)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1423 AA;  159660 MW;  C6148C019016F511 CRC64;
     MLPRTASATR TPHLNRLVSA GSRRVLPRCS SSLGIRGSLA ATPRLLSTIT TQQPSPLKRQ
     PAPRATVGFE RHLATVLDEP AQTATTPLYE LRSFSPQAPL TVRDHTKVYA KQRVNYHGIP
     GDVNEMFLVF EACLQVGRLE RAAQVLKRLA NLDVVPPADY LDLFNQYLDA KVSQLLQEPD
     VDKADDIHKA FETMVSDGRE LPVGGETVAL LLKASLTSTD PETMQRYVTR YLSLLPTQIA
     LETVFNTEIL TYEELTKVAE LCPKYNMPDN VDPDTFAQQQ QQQQQQQQQQ QEQQQQQDTS
     IDQSEVSIEP LLTSSEPSAI PEVLGTPQKG FGLQFVKRTV SMFKDIPDGF DISTLPMSQQ
     REIQSKLEKD CVDASLARWR EENESLQKMG LNTSLDTPSL NSRLYQWQKD LETRLRTMLV
     EVEKSEMVSK KNKDDLDRCI YGPFIRQSNP ERLAAVTIIS TLSSLAMGGA HKGSTIASLI
     THIAKFAEED IRVQKAEALI SKRNLRKAKS KQHNPRSVLR FKNSTASAGS SDMADSNNVA
     VEMDDEAWTT TIRTKVGAAL LSALLDTAKI TLVREDPVTK TLITQNQPAF SHVMQLRKGK
     KIGTIIPNKA VVELLVREPV PDFLARHLPM VTPPDPWVSF EKGAYLETKT PVLRLKNGER
     EQRLYTEAAI ARGDMDQVFK GLDVLGKTGW KINSPVFKVM LDVWNSGKQV ANIPPLDPIF
     DLPPEPASTE DPTVKRAWLK EIKVIENERS GLHSQRCFMN FQLEIARAYR DQTFYFPHNV
     DFRGRAYPIP PYLNHMGADH VRGLMLFAKG KPLGESGLRW LKVHLANVYG FDKASLQERQ
     DFADENIENI RDSVNNPLNG NQWWLQAEDP WQCLATCFEL AAALELEDPT KYVSHLPIHQ
     DGTCNGLQHY AALGGDTWGA QQVNLVPGDR PADVYSAVAK LVIKGIEDDL AKDNEFAKAM
     HGKITRKVVK QTVMTNVYGV TYVGARKQVL KQIEAAYPNI TAESGIEAAL LASYVTQHIF
     RAMSTMFKGA HDIQNWLGEI GGRVCRALTP EQLDEFERSE RSPHGDGTAS GENITLAGNP
     RKSSAHKNDE ILNNFQSTII WTTPLRMPVV QPYRKHGTKT VSTCMQDLVM TIPERSDPVN
     RRKQLQAFPP NFIHSLDASH MILSALHCDE LGLTFAAVHD SFWTHASDID SMNAVLRDAF
     IRIHSEDVIG RLAAEFQARY KNSLYLAKIE TGTKVAQEIQ RWRVRNKLGP RKELLLEKER
     QELLRSSNPE DVERGKKMIS PASLYELYSS AEDLTVPEDL KEVTIGNLAG VEETKVRRGR
     EMDEEGEVDG SEEAVEHEDG MHEDEMLADE PRDMDGNSGL DELSELRNTN HFALSQKRAK
     ASIASGGKQK HYLDIWLPLV FPPIPEKGDF DVRSLKDSTY FFS