RPOM_YEAST
ID RPOM_YEAST Reviewed; 1351 AA.
AC P13433; D6VTJ4;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=DNA-directed RNA polymerase, mitochondrial;
DE EC=2.7.7.6;
DE Flags: Precursor;
GN Name=RPO41; OrderedLocusNames=YFL036W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3308116; DOI=10.1016/0092-8674(87)90013-4;
RA Masters B.S., Stohl L.L., Clayton D.A.;
RT "Yeast mitochondrial RNA polymerase is homologous to those encoded by
RT bacteriophages T3 and T7.";
RL Cell 51:89-99(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC ECO:0000255|PROSITE-ProRule:PRU10032};
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M17539; AAA35007.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09203.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12404.1; -; Genomic_DNA.
DR PIR; S56218; S56218.
DR RefSeq; NP_116617.1; NM_001179930.1.
DR PDB; 6YMV; EM; 3.10 A; A=100-1351.
DR PDB; 6YMW; EM; 3.71 A; A=100-1351.
DR PDBsum; 6YMV; -.
DR PDBsum; 6YMW; -.
DR AlphaFoldDB; P13433; -.
DR SASBDB; P13433; -.
DR SMR; P13433; -.
DR BioGRID; 31110; 285.
DR ComplexPortal; CPX-3146; Mitochondrial DNA-directed RNA polymerase complex.
DR DIP; DIP-2431N; -.
DR IntAct; P13433; 7.
DR MINT; P13433; -.
DR STRING; 4932.YFL036W; -.
DR MaxQB; P13433; -.
DR PaxDb; P13433; -.
DR PRIDE; P13433; -.
DR EnsemblFungi; YFL036W_mRNA; YFL036W; YFL036W.
DR GeneID; 850507; -.
DR KEGG; sce:YFL036W; -.
DR SGD; S000001858; RPO41.
DR VEuPathDB; FungiDB:YFL036W; -.
DR eggNOG; KOG1038; Eukaryota.
DR GeneTree; ENSGT00390000008060; -.
DR HOGENOM; CLU_003364_1_0_1; -.
DR InParanoid; P13433; -.
DR OMA; PEKMCVI; -.
DR BioCyc; YEAST:G3O-30426-MON; -.
DR PRO; PR:P13433; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P13433; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IDA:SGD.
DR GO; GO:0005759; C:mitochondrial matrix; IDA:SGD.
DR GO; GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0003896; F:DNA primase activity; IDA:SGD.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IDA:SGD.
DR GO; GO:0001018; F:mitochondrial promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IDA:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0006390; P:mitochondrial transcription; IDA:SGD.
DR Gene3D; 1.10.1320.10; -; 1.
DR Gene3D; 1.10.287.260; -; 1.
DR InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR037159; RNA_POL_N_sf.
DR InterPro; IPR029262; RPOL_N.
DR PANTHER; PTHR10102; PTHR10102; 1.
DR Pfam; PF00940; RNA_pol; 1.
DR Pfam; PF14700; RPOL_N; 1.
DR SMART; SM01311; RPOL_N; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Mitochondrion;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT CHAIN ?..1351
FT /note="DNA-directed RNA polymerase, mitochondrial"
FT /id="PRO_0000031078"
FT ACT_SITE 945
FT /evidence="ECO:0000250"
FT ACT_SITE 1014
FT /evidence="ECO:0000250"
FT ACT_SITE 1189
FT /evidence="ECO:0000250"
FT CONFLICT 485
FT /note="G -> S (in Ref. 1; AAA35007)"
FT /evidence="ECO:0000305"
FT CONFLICT 900..901
FT /note="NP -> KS (in Ref. 1; AAA35007)"
FT /evidence="ECO:0000305"
FT HELIX 388..394
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 398..419
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 423..441
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 449..476
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 486..492
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 493..500
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 505..518
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 519..521
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 526..530
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 531..557
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 594..611
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 613..619
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 621..623
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 626..643
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 645..651
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 653..659
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 664..667
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 675..677
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 680..684
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 687..693
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 697..699
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 704..716
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 717..719
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 720..730
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 733..736
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 738..750
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 776..812
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 814..816
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 822..824
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 830..833
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 835..837
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 843..848
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 849..853
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 858..872
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 880..889
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 891..899
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 901..903
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 907..910
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 911..913
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 914..928
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 933..935
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 941..945
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 949..958
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 962..966
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 978..996
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 999..1004
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 1005..1007
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1010..1022
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1053..1065
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1067..1085
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1086..1088
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 1089..1091
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1096..1098
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1109..1111
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1117..1119
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1127..1131
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1136..1140
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1142..1144
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1150..1180
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1188..1194
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 1195..1197
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1198..1213
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1217..1228
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 1229..1231
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1232..1236
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1238..1240
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1247..1250
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1263..1275
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1303..1305
FT /evidence="ECO:0007829|PDB:6YMV"
FT TURN 1306..1308
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1309..1313
FT /evidence="ECO:0007829|PDB:6YMV"
FT STRAND 1323..1326
FT /evidence="ECO:0007829|PDB:6YMV"
FT HELIX 1340..1345
FT /evidence="ECO:0007829|PDB:6YMV"
SQ SEQUENCE 1351 AA; 153047 MW; 70A055CB71DB223E CRC64;
MLRPAYKSLV KTSLLQRRLI SSKGSKLFKP SPDSTSTILI SEDPLVTGSS PTSSTTSGII
SSNDFPLFNK NRKDAKSSIS YQWKNPSELE FDPFNKSHAS AVTSMTRTRD VMQLWSLLEA
CLQSNLMKRA FSILESLYLV PEHKQRFIED YNMYLNSFSK NDPNFPILKM NEKLTNDLET
SFKDVNYNDK TLAIMIHHAL NFHSTTSSML LKPIISAYLK MSVNGIREIF SCLDILTISD
LNILMNDLKV ITPSQLPNSV RPILESLTLS PTPVNNIENE EGLNKVEAEN DSKLHKASNA
SSDSIKKPSL DPLREVSFHG STEVLSKDAE KLIAVDTIGM RVIRHTLLGL SLTPEQKEQI
SKFKFDANDN VLKMKPTKND DNNNSINFFE IYNSLPTLEE KKAFESALNI FNQDRQKVLE
NRATEAARER WKHDFEEAKA RGDISIEKNL NVKLWKWYNE MLPLVKEEIN HCRSLLSEKL
SDKKGLNKVD TNRLGYGPYL TLIDPGKMCV ITILELLKLN STGGVIEGMR TARAVISVGK
AIEMEFRSEQ VLKSESQAFR DVNKKSPEFK KLVQNAKSVF RSSQIEQSKI LWPQSIRARI
GSVLISMLIQ VAKVSVQGVD PVTKAKVHGE APAFAHGYQY HNGSKLGVLK IHKTLIRQLN
GERLIASVQP QLLPMLVEPK PWVNWRSGGY HYTQSTLLRT KDSPEQVAYL KAASDNGDID
RVYDGLNVLG RTPWTVNRKV FDVVSQVWNK GEGFLDIPGA QDEMVLPPAP PKNSDPSILR
AWKLQVKTIA NKFSSDRSNR CDTNYKLEIA RAFLGEKLYF PHNLDFRGRA YPLSPHFNHL
GNDMSRGLLI FWHGKKLGPS GLKWLKIHLS NLFGFDKLPL KDRVAFTESH LQDIKDSAEN
PLTGDRWWTT ADKPWQALAT CFELNEVMKM DNPEEFISHQ PVHQDGTCNG LQHYAALGGD
VEGATQVNLV PSDKPQDVYA HVARLVQKRL EIAAEKGDEN AKILKDKITR KVVKQTVMTN
VYGVTYVGAT FQIAKQLSPI FDDRKESLDF SKYLTKHVFS AIRELFHSAH LIQDWLGESA
KRISKSIRLD VDEKSFKNGN KPDFMSSVIW TTPLGLPIVQ PYREESKKQV ETNLQTVFIS
DPFAVNPVNA RRQKAGLPPN FIHSLDASHM LLSAAECGKQ GLDFASVHDS YWTHASDIDT
MNVVLREQFI KLHEVDLVLR LKEEFDQRYK NYVKIGKLKR STDLAQKIIR IRKDLSRKLG
RSTTLADEIY FEKKRQELLN SPLIEDRNVG EKMVTTVSLF EDITDLDALE LENGGDENSG
MSVLLPLRLP EIPPKGDFDV TVLRNSQYFF S
