ID   RPOP_AGABT              Reviewed;        1102 AA.
AC   P33539;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
OS   Agaricus bitorquis (Pavement mushroom) (Psalliota bitorquis).
OG   Mitochondrion.
OG   Plasmid pEM.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Agaricaceae; Agaricus.
OX   NCBI_TaxID=5343;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 24666 / Ag4 / CBS 507.73;
RX   PubMed=1879001; DOI=10.1007/bf00312742;
RA   Robison M.M., Royer J.C., Horgen P.A.;
RT   "Homology between mitochondrial DNA of Agaricus bisporus and an internal
RT   portion of a linear mitochondrial plasmid of Agaricus bitorquis.";
RL   Curr. Genet. 19:495-502(1991).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X63075; CAA44799.1; -; Genomic_DNA.
DR   PIR; S28104; S28104.
DR   AlphaFoldDB; P33539; -.
DR   SMR; P33539; -.
DR   PRIDE; P33539; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..1102
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087753"
FT   ACT_SITE        734
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        804
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        980
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1102 AA;  126665 MW;  F72FDD308D1AB5B4 CRC64;
     NSIVRKRIEI STNENRGQLV VSSDKYSLVD NLFYNLHDYA SQSKHNIIID DGKVEKLLNL
     ICNVLDRNVN ELNDSVFLII KDIEKECKYY VSNVLYRSVG SRKNRGREVE WSSYKYNKEF
     NKVLDKGIIS INNEVLKFIS KEREGYIERV ESIAVTVKNK ILELNNNIAE VLLSIKNKVI
     VLNKESVVAK VEEINYEVHN KFIKGNGNTN FSNRNLTEIK SILKELNKME ILDNRINKLS
     TKESDLLKVI KEILDSNLII EDKQLAIEKT VVEYELTFFR HNMDTHETRN KIIHNIYPKL
     NKAYTELLAN YKLNRYSKIK KSIHLISNKS EGTKSKEMIK LIVVLVILYI GIDKCISYSF
     YQIINLLTNA RDGTSRTNIA INLGFRIIKV LKYIKLDENP SLNALYPINK LKDEISKLDN
     EGIYWIGDTL LGLITANCDI VVEELKWNSG KDSQLEVRIN DKFISNLTVS GINIVQLPML
     TEPRKISSDG LYFPYINSDT TNLHLFEGEL IKGKYNLRDH TEASEMLYSS INYLNSIKFK
     INKAMLNFIL AEWDNKDSKL FKGYNMLKPI LETDSKEIKE EKVSSNSKYT LYSNIISLAS
     LYKDNEFYLP VYVDFRGRVY PLSNYISYQG GDLARSLILF ADTKCVLNNS GKECLNVYLA
     NLAGYDKLPW SERLTKVDGI IKEYLESNEI SNTKYIEDNI DKISEPFQFI SIMYAKLLSI
     SNPKANISNP ILFDASCSGI QHIAALTLEK ELASNVNLYT DSSNPKEDYP QDFYTYALEK
     IRDKLINSDI TELRDIKLNR KIIKRSVMTI PYNISMAGIG EHLMEHFTVK TVLKYRYVVI
     PGSATISSKD VYLDYSKYGQ LCKIIYFVLT KELPSLRLLS NYFESMIDIF VKLNIPITWV
     TPSGLKIKYT NIKFKPQKVK TSVLNTSKIT TIKLPTDSLD VLSTKRSFMP NFIHSLDASN
     VHLLLNSVSY KNLPVYTVHD CFASTANNMF KLEKLVKNAF INIYFNDEGY LLKLHKHFVD
     TIISATDPYL SNGNIENEND IKGLTTERLE YKPLLSSNYV ADRISTKADI IKIPDLPAGY
     KNKNKNINEF VKGILNSKYF IG