ID   RPOP_CLAPU              Reviewed;         936 AA.
AC   P22372;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
DE   AltName: Full=ORF2;
OS   Claviceps purpurea (Ergot fungus) (Sphacelia segetum).
OG   Mitochondrion.
OG   Plasmid pClK1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX   NCBI_TaxID=5111;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K;
RX   PubMed=2770691; DOI=10.1007/bf00330952;
RA   Oeser B., Tudzynski P.;
RT   "The linear mitochondrial plasmid pClK1 of the phytopathogenic fungus
RT   Claviceps purpurea may code for a DNA polymerase and an RNA polymerase.";
RL   Mol. Gen. Genet. 217:132-140(1989).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; X15648; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JQ0302; JQ0302.
DR   AlphaFoldDB; P22372; -.
DR   SMR; P22372; -.
DR   PRIDE; P22372; -.
DR   VEuPathDB; FungiDB:CPUR_00077; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..936
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087754"
FT   ACT_SITE        576
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        654
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        839
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   936 AA;  107996 MW;  D51C1B9DC0F02EA1 CRC64;
     MLILLLQYNV SNPCLTNLLE NKTGTGVGLN FPLKPARLAL HGMGVRFYST MSSAKTTTTT
     LVTKSPKTKA TTMVTKSPFI FAHLQELLQN KPLNADLQVE IETFLDNQGE FLIQDHKYAE
     VDLNKVNSTM NNICFDKAEI MKKYLTKLKK SLNKASGLNK EGTTMKKKYD YLAYLVLRKV
     TPGVVVGLML YIFLLVVTYA NIYTDDKLHT GATIINIKMG KLIVDKYIQA LKGKKTLYSI
     FMSQFLLVED NKMFFDDAFY LHLGAKLIEI LIESDLLALT LVKFKDESHS VLTLTEEVAK
     AIPTNKVVLA PLKLPMIVAP KPYSKEEHGG YLLNDFDYLD SLLTKKLNMN DISRIQEKNI
     IYESINKMMA TAFQINKKLL KYLLEDYVFS KLLKLEGDDL YKNITQIEID NMDKRDNQKY
     QQYLSQKLLR DYIIDIATVF QNVPELYFPL KLDHRGRLYP ITAYFHYQSS ELAKALLLFA
     IPDTIERSDS ISIDYLKAYG ATCFGNKLDK KSYDKRLQWV DANWENIINY RNNVLLELAE
     NKFMFLAYCL EIERLNQFLN DDSIENFKTY LPLQLDGTCN GFQHIVLLSN EVKLYSQLNL
     DGKSKNQDPS DFYSFMINQL NIHLLNKKDV STDKEDIESY DRLINLGLSR ANIKQAIMTK
     PYNAKDFTVA KYIIETLLIA SEDKYIDEKG ITRTECWYKV NETMTNVACF RDIYLLAKCI
     NEILFLNYPQ IKSLLEYLNS ISEIMLKLNL PITWSLPTGL EVHQQYMRSK LKKTKPYTYL
     DTSLTLKITD KIKMDKVKQQ IALMPNLIHS LDAASLIMLY FAFNKSIGSG VVNFYSVHDC
     YGVTAKYIDL LISQLRAVYI ELYSKDGYIA HFDVDIINQI RKAYTNKKEI FTYDHKNRVI
     SINENIIVLP PIPNIGNQEH IRKAYEELAK ANMFIK