ID   RPOP_MAIZE              Reviewed;        1098 AA.
AC   P10581;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
DE   AltName: Full=S-2 DNA ORF1;
OS   Zea mays (Maize).
OG   Mitochondrion.
OG   Plasmid S-2.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16593334; DOI=10.1073/pnas.80.13.4055;
RA   Levings C.S. III, Sederoff R.R.;
RT   "Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of
RT   maize.";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:4055-4059(1983).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: The mitochondria from the S male-sterile cytoplasm of
CC       maize contain unique DNA-protein complexes, designated S-1 and S-2.
CC       These complexes consist of double-stranded linear DNAs with proteins
CC       covalently attached to the 5'-termini.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; J01426; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P10581; -.
DR   STRING; 4577.GRMZM5G827309_P01; -.
DR   PRIDE; P10581; -.
DR   MaizeGDB; 69623; -.
DR   eggNOG; KOG1038; Eukaryota.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..1098
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087756"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        663
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        750
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        915
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1098 AA;  128017 MW;  D448F26EC90FC314 CRC64;
     PIRESVRVST DRDPDLEDEK REQLGESMQT ELERLTWGVE VGTSEDINVD TVKRWGLQNN
     KYNAEHWIPP GGQRTAEDMG KLYQFWDEFI ETYENEVMMN SGYREPLNKD KVFNTLLDHS
     NNKTNQTAEE LKGIQTKIER MTMYFYEANV YESLGQLKNK FINIDEQTAK DYLLDKLEKP
     DDLDIVRAMG TYTLECIVVF VLSKQFNVFA LDKASVQVAT LVSELDSAAK IEYNRIIAED
     RKRKKAKWGD KQINEDENIL LQDKGVDTFN GDVHSELDRK KKSISKQTNR KRVRISKHKE
     PGIGEALFGW LASRKLIEVK KPVFLFDKKN KKPKNVIYPG YVDCLFDIKD LPFCSTLPMV
     YPPADWELWP TATAEVSDPY ITNLTPLSSY RGGYLTSLQR ESGDSPTLLS EKDYGVFDIH
     IDRERSQPVL SAVKKLQWQP YRINKLVYDF IQKHWSVLVS VGLLRPKNLA LFKRKEALRL
     LSSLLFKHEE LSTIYRYSEL KSVLLKNIHA STFELYTMKI AEAYLDYKIY FPIFLDFRGR
     NYRHGPFHFH ERDLVRSLII FDESDDSAAH TINSDVGDRI LHNFLISAAY HKSKFGVYRE
     ALEFIYNKIE DMQSKPTFFE KDIFVDTLCC RHPFQYISSC ISLKTYADTK DLSVLRYTPV
     FQDASASAYQ IMSYFLLDID YGIHTNLLKK TNTDGRYIRD IYEFMWGCLI KYLIAEEKIE
     LAIKLLTPNE KDQESVLAKI VSIFDRNVVK KMFMPMMYGK TDYTLKKDVE DLLKGKSDSE
     GINLISKHIS TYWKVNFGKM KDLMDLINYV SWFGAGQDKP VVYSTPYWVT LQTYKWRKRV
     KMKIQYETTK NNEKEVKTTS AKMLIPLNDN DIRKSSTSTF ANFIHQKDAF TAIQLVDFIN
     KLENASSIPI YAVHDNFITM PEYASILPTL YRDSIFRMGH PLIIINKFLF DHILIPAIQN
     EHPQNKHLFS VEERSMLDRM MIDLQNPLIP DFGSVDITKA RIKSIVIPKD LLLKCFSCLW
     MSKTKKISLV RWESCRDKII KVYMRYTDDI SSDEGVSRWL EYKNNLEFAS DPVWSSDNTN
     GTQADSLDKG EDDYCIHY