ID   RPOP_NEUCS              Reviewed;         896 AA.
AC   P33540;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
OS   Neurospora crassa.
OG   Mitochondrion.
OG   Plasmid maranhar.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=5141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AAREY-1E;
RX   PubMed=1423726; DOI=10.1007/bf00352440;
RA   Court D.A., Bertrand H.;
RT   "Genetic organization and structural features of maranhar, a senescence-
RT   inducing linear mitochondrial plasmid of Neurospora crassa.";
RL   Curr. Genet. 22:385-397(1992).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X55361; CAA39045.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S26984; S26984.
DR   AlphaFoldDB; P33540; -.
DR   SMR; P33540; -.
DR   VEuPathDB; FungiDB:NCU06308; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..896
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087757"
FT   ACT_SITE        546
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        617
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        798
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   896 AA;  103973 MW;  112FD0C68225A4D8 CRC64;
     MIKFYFTTFH MKFPARLFST SFTLNNTNII NEKDVKYITK NFLLENSNGF NLIKNIINSD
     ETSEVKQNKI EVELNNIWHT EITDILQKKR SLGLDAIGSS ILAKDFHNLI GDIENFINKG
     RTNKLPGVEY LKPSLIISIV LGKVIPFSLR HSDIFNQPTH SLFAEIGKIL KYQSIFELHH
     RIGLIQNRVE EIKNYTEKKL VSEFNKLTKV LDEYKNTLKN LEELSGESII KVGLGFTALL
     SETSEFYSLE EQIIAKNKSI RYIIPKNKLK TLINNITLMD TIELPMIIPP LEWKIDDNEK
     IIEYGGTILN NKHRIRPLRT KSVENSDAND MTYNKELVDA VNFKSKIPYT INLKILDFIT
     RDEFINRDKK DNVIIYKHIH PDSALLGEYM KDRKNPKISE ITTHNSKFLY HSSIISIAKL
     MKDVKEFYMT VFIDWRGRFY TSSCALNIQG GELARSLLLF KEGQKLNDIG LKALKIYTAN
     AFGLDKRSKE ERLDWVEQNL HKIIDIDNYE IWREADEPLL FLACALELKG YNEDPNFISH
     LPILMDATCN GLQHLSAMVN DFVLAEKVNL LKSTENDNPR DLYSEVIPHI KQEILEASKS
     YEHTNLERIN VERCLVKRGL MTITYGATER GIYDQIVSKF FQKDEWNKTV GLHFVCIDSD
     IAPKDVVFTQ KNILLLSKII YNSLFKIHPN LNTLMVYFNS IVKVLCELDL PVNWVTPYGL
     VIQQKYNKFT KYNETTYVAS KRYKLVLRKA DTTSISKQKQ IQAFIPNFVH SMDGSNIVLL
     INTIRDEGRK INFASIHDCF ATHANDTAWL SWYVKQSFIR IYSDSSFLRR FHNYIQLRIQ
     AKYGEDAHFD KDGVLTHVTI DNGTDIPDRI DIPKPLVVNK DNKIYKEILH SEYFIN