ID   RPOP_NEUIN              Reviewed;         811 AA.
AC   P33541;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   04-MAY-2001, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
OS   Neurospora intermedia.
OG   Mitochondrion.
OG   Plasmid kalilo.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=5142;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1934129; DOI=10.1007/bf00326237;
RA   Chan B.S.S., Court D.A., Vierula P.J., Bertrand H.;
RT   "The kalilo linear senescence-inducing plasmid of Neurospora is an
RT   invertron and encodes DNA and RNA polymerases.";
RL   Curr. Genet. 20:225-237(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
RX   PubMed=1406582; DOI=10.1007/bf00538695;
RA   Vierula P.J., Bertrand H.;
RT   "A deletion derivative of the kalilo senescence plasmid forms hairpin and
RT   duplex DNA structures in the mitochondria of Neurospora.";
RL   Mol. Gen. Genet. 234:361-368(1992).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; X52106; CAA36326.1; -; Genomic_DNA.
DR   PIR; S17908; S17908.
DR   AlphaFoldDB; P33541; -.
DR   SMR; P33541; -.
DR   PRIDE; P33541; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..811
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087758"
FT   ACT_SITE        470
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        540
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        718
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   811 AA;  94801 MW;  286225A36B625BBC CRC64;
     MSKFSFFNYR INMRNSHATS LRFIKFNYSE FKNIRNFSSE KKRDPKLSLF LNNVSKILSE
     HKKDLHKAQE IIEREWLKLA TPSTDVKSDI TSTMYTKHRL IHDRAFDLLK LYKSNGNLNK
     ISKSLKDYIV NEKFIFIAWS YLLASYSKSS LTNLDYRIGY SITRHIYTEY YKDEFMSFDD
     FSNYNKFDVG FFVKLGDIFI NTFTSPLNPI FERVFENEIY SLKINKEYEN EIMESLVISP
     KALPMVCPPL PWGHGIRGGN LINLTDTQDE SLVVGSFHHR HKIAITDKLY NVINKLNAFK
     FKINGDLLSY LQNEGSFILD FYKNTKKDTY INNMITIDIA RTYLNTPFYL NVNIDWRGRI
     YTQSFYLDYQ GSELSLALIN LFEGKKLDEK GLFFFYVYGA NIYNDGGKFS KKSFQDRFNW
     VVENLDNIIA MDKEFILKAE SPTLFAAFCL TMRKLKENPD YPVFNPIFLD ATCSGVQHFA
     AMLLDLELGK YVNLINSGES VNDFYSQLIP AINKAINESA EKKFKNLKFS DISLNRSLLK
     KVIMTKSYNV TTYGITEQLK SKLEKVEKIV ISKGKEIKVY DYLVPTKNGD FVVLDTFEVE
     TLASIINDNI FNQFPKLHSI YDYLTRLAKI YLKLDIPLSW STPDGLELTQ RYNLSKVKKL
     TINFLGKNRT AVLRSWVNEK DSRREVQAII PNIIHSLDAS HLTMIIDSWD SYILPIHECF
     GTHPNDMYKL AEQVRECFIL LYSKNDFLNK IDYKFRENLK DYKIEIVNKN GEDFVKIKGN
     KRYEYLPLPV LPQMGELNVE DIRDMGKYMI S