ID   RPOP_PODAS              Reviewed;         948 AA.
AC   Q01521;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Probable DNA-directed RNA polymerase;
DE            EC=2.7.7.6;
OS   Podospora anserina (Pleurage anserina).
OG   Mitochondrion.
OG   Plasmid pAL2-1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Podosporaceae; Podospora.
OX   NCBI_TaxID=2587412;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AL2;
RX   PubMed=1473181; DOI=10.1007/bf00326415;
RA   Hermanns J., Osiewacz H.D.;
RT   "The linear mitochondrial plasmid pAL2-1 of a long-lived Podospora anserina
RT   mutant is an invertron encoding a DNA and RNA polymerase.";
RL   Curr. Genet. 22:491-500(1992).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; X60707; CAA43116.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q01521; -.
DR   SMR; Q01521; -.
DR   VEuPathDB; FungiDB:PODANS_6_9830; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..948
FT                   /note="Probable DNA-directed RNA polymerase"
FT                   /id="PRO_0000087759"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        680
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        853
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   948 AA;  110729 MW;  BB92D532C3B06C6A CRC64;
     MKKNNTILYY GCAAQARYAS SNHGLLYSPS QAHLFNFSLA RTYLNFSNGF RAFSTSLHLS
     YNQPLITYDL QDHTIDIEEM KKENLEKSFK SNTYIFKCLL NTINNKPINE KTQMEIEELL
     NKFTYASFSK KLEANQKNPE IIDYSLLNPK LAAILTDVRP TLISIINNLQ KDYPDYFMAK
     LFKKSDKNEY YIGLVIKGLT PEEIINSIFF HVLKVLSFHT KKLDKSNSTS DIAFGMGKVL
     VENFYYNEFI KIKNKLSKEE QKCYYLSNWK KDNKDIVEKL EDNIFQFLLG QKISNLTKDT
     DLLDMETVTM AYREKSIVWK PTKKILKVLP EDGTLFVLPK KIPMIVKPKP YTTRVNGGYL
     SNDLYINENI VKEKWNLRDN TQILHFNQIF DLVNNLSSIG YKINTDVLDF IELYGSDYFK
     NELIDPRYSH PLLKKSKLTK KEKMELDSFL AKKELQENIL GLAEIFRNIP EFFIPVNADF
     RGRVYCTPEY LNYQSTDLAK SLLLFSKPGR MYKKDYIALS YLKIYGGSSF GLDKLSANDR
     IKWVDKNLNN IKNYRNGKLI KEAKNKFLFL AFCIEYNRYL NCLDNHDVSW FDTYLPIQMD
     ATCNGFQHLS LLSLDSNLSK ELNLSESTWD DVPKDFYTFL VVCFIDYLKT ELLENKNLTP
     KESESYNRLI NMKIIREIIK KGIMTIPYNV SNFSLINYIR EGFELEDNSL EWYIYKNEPS
     IRLKSLDFTV LGKGLRKVLH EKFHKLDLLL KYLDQVARVC TLLEIPIIWT LPTGLVVRQS
     YMIRDEVRIK PFNHSNKKFS MKVLNKTQFN NSKQITAFMP NLVHSLDAAS LTLLLDFYFK
     ESIDVKNIYT IHDCFAVPAN KMECLISLLK LTYIKLYSDD KYLLKLDDDI RKNIRSTQNK
     GCFNEETLEI TGDSFPDPIK FPDVQKVIGI VPSDFDFNVL KKSSYILN