RPOS_COXBU
ID RPOS_COXBU Reviewed; 352 AA.
AC Q9KI19; Q7C3E6;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; OrderedLocusNames=CBU_1669;
OS Coxiella burnetii (strain RSA 493 / Nine Mile phase I).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC Coxiella.
OX NCBI_TaxID=227377;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=RSA 493 / Nine Mile phase I;
RX PubMed=11447163; DOI=10.1128/iai.69.8.4874-4883.2001;
RA Seshadri R., Samuel J.E.;
RT "Characterization of a stress-induced alternate sigma factor, RpoS, of
RT Coxiella burnetii and its expression during the development cycle.";
RL Infect. Immun. 69:4874-4883(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RSA 493 / Nine Mile phase I;
RX PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C.,
RA Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T.,
RA Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M.,
RA Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E.,
RA Fraser C.M., Heidelberg J.F.;
RT "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- DEVELOPMENTAL STAGE: Found in the large cell variant (LCV) stage, very
CC little is present in the small cell variant (SCV) stage (at protein
CC level). LCVs are more metabolically active than SCVs.
CC {ECO:0000269|PubMed:11447163}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF244357; AAF61172.1; -; Genomic_DNA.
DR EMBL; AE016828; AAO91165.1; -; Genomic_DNA.
DR RefSeq; NP_820651.1; NC_002971.3.
DR RefSeq; WP_010958362.1; NC_002971.4.
DR AlphaFoldDB; Q9KI19; -.
DR SMR; Q9KI19; -.
DR STRING; 227377.CBU_1669; -.
DR EnsemblBacteria; AAO91165; AAO91165; CBU_1669.
DR GeneID; 1209580; -.
DR KEGG; cbu:CBU_1669; -.
DR PATRIC; fig|227377.7.peg.1659; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; RRVQREF; -.
DR Proteomes; UP000002671; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..352
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000318558"
FT DNA_BIND 311..330
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..112
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 117..187
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 197..272
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 285..338
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 141..144
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 1..37
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 352 AA; 40471 MW; 35EA31D1C1132251 CRC64;
MKTKTTKKTI KKAAKKIKKP SKRKIKKTAK KSRPKKPIKA SDHGLIFAKT KKETTEKEDA
ELANAKAKTK KRRETRSSDP TQIYLRELGF QPLLNAKEEL KIARRVHKGD PKARKQMIEA
NLRLVVKIAR HYVNRGLPFL DLIEEGNLGL LTAVEKFDPE RGFRFSTYAT WWIRQTIERA
IMNQSRTVRL PIHVIKELNV YLRTAKKLTQ EVDHEATPED VAHLIDKPVQ EIRRIMDLAP
SATSIDVPIS EDGQKSLVDT LADDNNIDPA RLIQNVDLQD HIERWLAQLD ERHREVVILR
FGLHENEKGT LEAVGKAVGL TRERVRQIQI DALQQLRHIL EMEGVTGEEV ED
