RPOS_PSEAE
ID RPOS_PSEAE Reviewed; 334 AA.
AC P45684;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; OrderedLocusNames=PA3622;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=7959068; DOI=10.1016/0378-1119(94)90862-1;
RA Tanaka K., Takahashi H.;
RT "Cloning, analysis and expression of an rpoS homologue gene from
RT Pseudomonas aeruginosa PAO1.";
RL Gene 150:81-85(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
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DR EMBL; D26134; BAA05131.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07010.1; -; Genomic_DNA.
DR PIR; S55064; S55064.
DR RefSeq; NP_252312.1; NC_002516.2.
DR RefSeq; WP_003113871.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; P45684; -.
DR SMR; P45684; -.
DR STRING; 287.DR97_4317; -.
DR PaxDb; P45684; -.
DR PRIDE; P45684; -.
DR EnsemblBacteria; AAG07010; AAG07010; PA3622.
DR GeneID; 880421; -.
DR KEGG; pae:PA3622; -.
DR PATRIC; fig|208964.12.peg.3791; -.
DR PseudoCAP; PA3622; -.
DR HOGENOM; CLU_014793_3_5_6; -.
DR InParanoid; P45684; -.
DR OMA; RRVQREF; -.
DR PhylomeDB; P45684; -.
DR BioCyc; PAER208964:G1FZ6-3692-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR GO; GO:1900377; P:negative regulation of secondary metabolite biosynthetic process; IMP:PseudoCAP.
DR GO; GO:0050921; P:positive regulation of chemotaxis; IMP:PseudoCAP.
DR GO; GO:1900233; P:positive regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:2000145; P:regulation of cell motility; IMP:PseudoCAP.
DR GO; GO:1900034; P:regulation of cellular response to heat; IMP:PseudoCAP.
DR GO; GO:1900407; P:regulation of cellular response to oxidative stress; IMP:PseudoCAP.
DR GO; GO:1901000; P:regulation of response to salt stress; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:PseudoCAP.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..334
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000093975"
FT DNA_BIND 293..312
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 21..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..94
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 99..169
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 179..254
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 267..320
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 123..126
FT /note="Interaction with polymerase core subunit RpoC"
FT COMPBIAS 32..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 334 AA; 38235 MW; B049427E5CBDC9DD CRC64;
MALKKEGPEF DHDDEVLLLE PGIMLDESSA DEQPSPRATP KATTSFSSKQ HKHIDYTRAL
DATQLYLNEI GFSPLLTPEE EVHFARLAQK GDPAGRKRMI ESNLRLVVKI ARRYVNRGLS
LLDLIEEGNL GLIRAVEKFD PERGFRFSTY ATWWIRQTIE RAIMNQTRTI RLPIHVVKEL
NVYLRAAREL THKLDHEPSP EEIANLLEKP VAEVKRMLGL NERVTSVDVS LGPDSDKTLL
DTLTDDRPTD PCELLQDDDL SESIDQWLTE LTDKQREVVI RRFGLRGHES STLEEVGQEI
GLTRERVRQI QVEALKRLRE ILEKNGLSSD ALFQ
