RPOS_SALT1
ID RPOS_SALT1 Reviewed; 330 AA.
AC D0ZVL4; P0A2E5; P37400; P39699; Q56132; Q93V26; Q9RN87; Q9RN91; Q9RN92;
AC Q9RN93;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; Synonyms=katF;
GN OrderedLocusNames=STM14_3526;
OS Salmonella typhimurium (strain 14028s / SGSC 2262).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=588858;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=14028s / SGSC 2262;
RX PubMed=8086465; DOI=10.1016/0167-4781(94)90271-2;
RA Prince R.W., Fang F.C., Libby S.J.;
RT "Cloning and sequencing of the gene encoding the RpoS (KatF) sigma factor
RT from Salmonella typhimurium 14028s.";
RL Biochim. Biophys. Acta 1219:198-200(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=14028s / SGSC 2262;
RX PubMed=19897643; DOI=10.1128/jb.01233-09;
RA Jarvik T., Smillie C., Groisman E.A., Ochman H.;
RT "Short-term signatures of evolutionary change in the Salmonella enterica
RT serovar typhimurium 14028 genome.";
RL J. Bacteriol. 192:560-567(2010).
RN [3]
RP MUTAGENESIS OF 61-TYR--ILE-65.
RC STRAIN=14028s / SGSC 2262;
RX PubMed=16763111; DOI=10.1126/science.1128794;
RA Navarre W.W., Porwollik S., Wang Y., McClelland M., Rosen H., Libby S.J.,
RA Fang F.C.;
RT "Selective silencing of foreign DNA with low GC content by the H-NS protein
RT in Salmonella.";
RL Science 313:236-238(2006).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
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DR EMBL; U05011; AAA61628.1; -; Genomic_DNA.
DR EMBL; CP001363; ACY89939.1; -; Genomic_DNA.
DR RefSeq; WP_000081498.1; NZ_CP043402.1.
DR AlphaFoldDB; D0ZVL4; -.
DR SMR; D0ZVL4; -.
DR EnsemblBacteria; ACY89939; ACY89939; STM14_3526.
DR GeneID; 58317824; -.
DR GeneID; 64173183; -.
DR GeneID; 67517453; -.
DR KEGG; seo:STM14_3526; -.
DR PATRIC; fig|588858.6.peg.3242; -.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; RRVQREF; -.
DR BioCyc; SENT588858:STM14_RS15660-MON; -.
DR Proteomes; UP000002695; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..330
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000424539"
FT DNA_BIND 288..307
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 56..89
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 94..164
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 174..249
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 262..315
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 118..121
FT /note="Interaction with polymerase core subunit RpoC"
FT MUTAGEN 61..65
FT /note="Missing: Reduced sigma activity, permits viability
FT of hns deletion strains."
FT /evidence="ECO:0000269|PubMed:16763111"
FT CONFLICT 83
FT /note="A -> D (in Ref. 1; AAA61628)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 330 AA; 37933 MW; 4400E48DEB358ECC CRC64;
MSQNTLKVHD LNEDAEFDEN GVEAFDEKAL SEEEPSDNDL AEEELLSQGA TQRVLDATQL
YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI
EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR
TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD
EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
RVRQIQVEGL RRLREILQTQ GLNIEALFRE
