ID   RPOS_SALTI              Reviewed;         330 AA.
AC   P0A2E6; P37400; P39699; Q56132; Q93V26; Q9RN87; Q9RN91; Q9RN92; Q9RN93;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE   AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE   AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN   Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; Synonyms=katF;
GN   OrderedLocusNames=STY3049, t2825;
OS   Salmonella typhi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ty21a;
RX   PubMed=7705608; DOI=10.1111/j.1574-6968.1995.tb07412.x;
RA   Robbe-Saule V., Coynault C., Norel F.;
RT   "The live oral typhoid vaccine Ty21a is a rpoS mutant and is susceptible to
RT   various environmental stresses.";
RL   FEMS Microbiol. Lett. 126:171-176(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2;
RX   PubMed=9919662; DOI=10.1111/j.1574-6968.1999.tb13366.x;
RA   Robbe-Saule V., Norel F.;
RT   "The rpoS mutant allele of Salmonella typhi Ty2 is identical to that of the
RT   live typhoid vaccine Ty21a.";
RL   FEMS Microbiol. Lett. 170:141-143(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT18;
RX   PubMed=11677608; DOI=10.1038/35101607;
RA   Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA   Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA   Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA   Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA   Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA   Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A.,
RA   Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA   Barrell B.G.;
RT   "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT   serovar Typhi CT18.";
RL   Nature 413:848-852(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2;
RX   PubMed=12644504; DOI=10.1128/jb.185.7.2330-2337.2003;
RA   Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V.,
RA   Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT   "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT   CT18.";
RL   J. Bacteriol. 185:2330-2337(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the master transcriptional regulator of
CC       the stationary phase and the general stress response.
CC       {ECO:0000255|HAMAP-Rule:MF_00959}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00959}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00959}.
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DR   EMBL; X81641; CAA57298.1; -; Genomic_DNA.
DR   EMBL; Y17610; CAA76807.1; -; Genomic_DNA.
DR   EMBL; AL513382; CAD06030.1; -; Genomic_DNA.
DR   EMBL; AE014613; AAO70382.1; -; Genomic_DNA.
DR   PIR; AG0855; AG0855.
DR   PIR; S58446; S58446.
DR   RefSeq; NP_457313.1; NC_003198.1.
DR   RefSeq; WP_000081498.1; NZ_WSUR01000005.1.
DR   AlphaFoldDB; P0A2E6; -.
DR   SMR; P0A2E6; -.
DR   IntAct; P0A2E6; 1.
DR   STRING; 220341.16503997; -.
DR   EnsemblBacteria; AAO70382; AAO70382; t2825.
DR   GeneID; 58317824; -.
DR   GeneID; 64173183; -.
DR   GeneID; 67517453; -.
DR   KEGG; stt:t2825; -.
DR   KEGG; sty:STY3049; -.
DR   PATRIC; fig|220341.7.peg.3101; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_5_6; -.
DR   OMA; RRVQREF; -.
DR   Proteomes; UP000000541; Chromosome.
DR   Proteomes; UP000002670; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   HAMAP; MF_00959; Sigma70_RpoS; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..330
FT                   /note="RNA polymerase sigma factor RpoS"
FT                   /id="PRO_0000093972"
FT   DNA_BIND        288..307
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          56..89
FT                   /note="Sigma-70 factor domain-1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          94..164
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          174..249
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          262..315
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   MOTIF           118..121
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   VARIANT         312..330
FT                   /note="RLREILQTQGLNIEALFRE -> PSARNSADAGAEYRSAVPRVSTLVKKRPV
FT                   DRLAFFLPFVFCQQRRDTRHHWRVINIFLRHAKRLWIVFAAGTA (in strain:
FT                   Ty2 and Ty21a)"
SQ   SEQUENCE   330 AA;  37933 MW;  4400E48DEB358ECC CRC64;
     MSQNTLKVHD LNEDAEFDEN GVEAFDEKAL SEEEPSDNDL AEEELLSQGA TQRVLDATQL
     YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI
     EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR
     TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD
     EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
     RVRQIQVEGL RRLREILQTQ GLNIEALFRE