ID   RPOS_SALTU              Reviewed;         330 AA.
AC   F5ZTT9;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE   AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE   AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN   Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; OrderedLocusNames=STMUK_2913;
OS   Salmonella typhimurium (strain ATCC 68169 / UK-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=990282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 68169 / UK-1;
RX   PubMed=21622747; DOI=10.1128/jb.05224-11;
RA   Luo Y., Kong Q., Yang J., Golden G., Wanda S.Y., Jensen R.V., Ernst P.B.,
RA   Curtiss R. III;
RT   "Complete genome sequence of the universal killer Salmonella enterica
RT   serovar typhimurium UK-1 (ATCC 68169).";
RL   J. Bacteriol. 193:4035-4036(2011).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 68169 / UK-1;
RX   PubMed=9573193; DOI=10.1128/jb.180.9.2409-2417.1998;
RA   Bearson B.L., Wilson L., Foster J.W.;
RT   "A low pH-inducible, PhoPQ-dependent acid tolerance response protects
RT   Salmonella typhimurium against inorganic acid stress.";
RL   J. Bacteriol. 180:2409-2417(1998).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the master transcriptional regulator of
CC       the stationary phase and the general stress response, including
CC       response to organic acid stress. {ECO:0000255|HAMAP-Rule:MF_00959,
CC       ECO:0000269|PubMed:9573193}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00959}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC   -!- DISRUPTION PHENOTYPE: Very sensitive to pH 3.0 when grown on glucose;
CC       tolerance increases 10,000-fold in a pgi disruption.
CC       {ECO:0000269|PubMed:9573193}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00959}.
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DR   EMBL; CP002614; AEF08778.1; -; Genomic_DNA.
DR   RefSeq; WP_000081498.1; NC_016863.1.
DR   AlphaFoldDB; F5ZTT9; -.
DR   SMR; F5ZTT9; -.
DR   GeneID; 58317824; -.
DR   GeneID; 64173183; -.
DR   GeneID; 67517453; -.
DR   KEGG; sej:STMUK_2913; -.
DR   PATRIC; fig|990282.4.peg.3039; -.
DR   HOGENOM; CLU_014793_3_5_6; -.
DR   Proteomes; UP000008278; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   HAMAP; MF_00959; Sigma70_RpoS; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..330
FT                   /note="RNA polymerase sigma factor RpoS"
FT                   /id="PRO_0000424540"
FT   DNA_BIND        288..307
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          56..89
FT                   /note="Sigma-70 factor domain-1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          94..164
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          174..249
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   REGION          262..315
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT   MOTIF           118..121
FT                   /note="Interaction with polymerase core subunit RpoC"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
SQ   SEQUENCE   330 AA;  37933 MW;  4400E48DEB358ECC CRC64;
     MSQNTLKVHD LNEDAEFDEN GVEAFDEKAL SEEEPSDNDL AEEELLSQGA TQRVLDATQL
     YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI
     EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR
     TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD
     EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
     RVRQIQVEGL RRLREILQTQ GLNIEALFRE